Endogenous hydrogen peroxide increases biofilm formation by inducing exopolysaccharide production in Acinetobacter oleivorans DR1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, In-Ae | - |
dc.contributor.author | Kim, Jisun | - |
dc.contributor.author | Park, Woojun | - |
dc.date.accessioned | 2021-09-04T02:46:23Z | - |
dc.date.available | 2021-09-04T02:46:23Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-02-17 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/89502 | - |
dc.description.abstract | In this study, we investigated differentially expressed proteins in Acinetobacter oleivorans cells during planktonic and biofilm growth by using 2-dimensional gel electrophoresis combined with matrix-assisted laser desorption time-of-flight mass spectrometry. We focused on the role of oxidative stress resistance during biofilm formation using mutants defective in alkyl hydroperoxide reductase (AhpC) because its production in aged biofilms was enhanced compared to that in planktonic cells. Results obtained using an ahpC promoter-gfp reporter vector showed that aged biofilms expressed higher ahpC levels than planktonic cells at 48 h. However, at 24 h, ahpC expression was higher in planktonic cells than in biofilms. Deletion of ahpC led to a severe growth defect in rich media that was not observed in minimal media and promoted early biofilm formation through increased production of exopolysaccharide (EPS) and EPS gene expression. Increased endogenous H2O2 production in the ahpC mutant in rich media enhanced biofilm formation, and this enhancement was not observed in the presence of antioxidants. Exogenous addition of H2O2 promoted biofilm formation in wild type cells, which suggested that biofilm development is linked to defense against H2O2. Collectively, our data showed that EPS production caused by H2O2 stress enhances biofilm formation in A. oleivorans. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | ALKYL HYDROPEROXIDE REDUCTASE | - |
dc.subject | SP STRAIN DR1 | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | PSEUDOMONAS-AERUGINOSA | - |
dc.subject | STAPHYLOCOCCUS-EPIDERMIDIS | - |
dc.subject | HEXADECANE DEGRADATION | - |
dc.subject | SALMONELLA-TYPHIMURIUM | - |
dc.subject | MOLECULAR-MECHANISMS | - |
dc.subject | PLANKTONIC CELLS | - |
dc.subject | STRESS RESPONSES | - |
dc.title | Endogenous hydrogen peroxide increases biofilm formation by inducing exopolysaccharide production in Acinetobacter oleivorans DR1 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jisun | - |
dc.contributor.affiliatedAuthor | Park, Woojun | - |
dc.identifier.doi | 10.1038/srep21121 | - |
dc.identifier.scopusid | 2-s2.0-84958576885 | - |
dc.identifier.wosid | 000370381100001 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.6 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 6 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | ALKYL HYDROPEROXIDE REDUCTASE | - |
dc.subject.keywordPlus | SP STRAIN DR1 | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PSEUDOMONAS-AERUGINOSA | - |
dc.subject.keywordPlus | STAPHYLOCOCCUS-EPIDERMIDIS | - |
dc.subject.keywordPlus | HEXADECANE DEGRADATION | - |
dc.subject.keywordPlus | SALMONELLA-TYPHIMURIUM | - |
dc.subject.keywordPlus | MOLECULAR-MECHANISMS | - |
dc.subject.keywordPlus | PLANKTONIC CELLS | - |
dc.subject.keywordPlus | STRESS RESPONSES | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.