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AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells

Authors
Kim, NamiLee, Jung OkLee, Hye JeongLee, Yong WooKim, Hyung IpKim, Su JinPark, Sun HwaLee, Chul SuRyoo, Sun WooHwang, Geum-SookKim, Hyeon Soo
Issue Date
2월-2016
Publisher
BIOSCIENTIFICA LTD
Keywords
AMPK; isoeugenol; glucose uptake; p38 MAPK
Citation
JOURNAL OF ENDOCRINOLOGY, v.228, no.2, pp.105 - 114
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF ENDOCRINOLOGY
Volume
228
Number
2
Start Page
105
End Page
114
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/89629
DOI
10.1530/JOE-15-0302
ISSN
0022-0795
Abstract
Isoeugenol exerts various beneficial effects on human health. However, the mechanisms underlying these effects are poorly understood. In this study, we observed that isoeugenol activated AMP-activated protein kinase (AMPK) and increased glucose uptake in rat L6 myotubes. Isoeugenol-induced increase in intracellular calcium concentration and glucose uptake was inhibited by STO-609, an inhibitor of calcium/calmodulin-dependent protein kinase kinase (CaMKK). Isoeugenol also increased the phosphorylation of protein kinase C-alpha (PKC alpha). Chelation of calcium with BAPTA-AM blocked isoeugenol-induced AMPK phosphorylation and glucose uptake. Isoeugenol stimulated p38MAPK phosphorylation that was inhibited after pretreatment with compound C, an AMPK inhibitor. Isoeugenol also increased glucose transporter type 4 (GLUT4) expression and its translocation to the plasma membrane. GLUT4 translocation was not observed after the inhibition of AMPK and CaMKK. In addition, isoeugenol activated the Akt substrate 160 (AS160) pathway, which is downstream of the p38MAPK pathway. Knockdown of the gene encoding AS160 inhibited isoeugenol-induced glucose uptake. Together, these results indicate that isoeugenol exerts beneficial health effects by activating the AMPK/p38MAPK/AS160 pathways in skeletal muscle.
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