Stimulatory effects of interleukin-1 beta on development of porcine uterine epithelial cell are mediated by activation of the ERK1/2 MAPK cell signaling cascade
DC Field | Value | Language |
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dc.contributor.author | Jeong, Wooyoung | - |
dc.contributor.author | Kim, Jinhyeon | - |
dc.contributor.author | Bazer, Fuller W. | - |
dc.contributor.author | Song, Gwonhwa | - |
dc.contributor.author | Kim, Jinyoung | - |
dc.date.accessioned | 2021-09-04T04:12:30Z | - |
dc.date.available | 2021-09-04T04:12:30Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-01-05 | - |
dc.identifier.issn | 0303-7207 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/89834 | - |
dc.description.abstract | Successful establishment of pregnancy depends on timely changes in the conceptus (embryo and associated extra-embryonic membranes) and uterine endometrium orchestrated by molecules from both the conceptus and uterus. Interleukin-1 beta (IL-1 beta) is an important mediator of that communication regulating development of the peri-implantation conceptus and opening the window of implantation during early pregnancy. However, little is known about IL-1 beta-mediated intracellular signaling cascades and functional effects in uterine luminal epithelium (LE) during the peri-implantation period of pregnancy in pigs. Therefore, this study determined, using an immortalized porcine LE (pLE) cell line from day 12 pregnant gilts: 1) the intracellular signaling cascade responsible for activities of IL-1 beta in pLE cells, and 2) the changes in cellular activities induced by IL-1 beta. IL-1 beta stimulated phosphorylation of ERK1/2 proteins in pLE cells in a dose-dependent manner. Ten ng/ml IL-1 beta increased levels of phosphorylated (p)-ERK1/2 proteins in pLE cells within 15 min post-treatment, and this IL-1 beta-induced phosphorylated status was inhibited by increasing doses of U0126 (ERK1/2 inhibitor). In addition IL-10 increased p-P7056K, pP90S6K, p-S6, and p-P38 proteins in a time-dependent manner, but IL-1 beta-induced activation of P70S6K and S6 proteins was significantly decreased in the presence of pharmacological inhibitors for ERK1/2 (U0126), MTOR (rapamycin), and P38 (SB203580). Moreover, IL-1 beta treatment potently increased the abundance of p-ERK1/2 proteins in the nucleus and cytoplasm. Similarly cytoplasmic p-S6 proteins were localized abundantly in the pLE cells treated with IL-1 beta. Furthermore, IL-10 increased proliferation of pLE cells by approximately 200%, and pretreatment of pLE cells with U0126 significantly inhibited this stimulatory effect. Collectively, results of this study indicate that IL-1 beta plays an important role in development of uterine LE by stimulating cell proliferation, and that these effects are coordinately regulated by activation of the ERK1/2 and P38 MAPK cell signaling cascades. (C) 2015 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | FACTOR-KAPPA-B | - |
dc.subject | PREIMPLANTATION MOUSE EMBRYOS | - |
dc.subject | ENDOMETRIAL GLANDS | - |
dc.subject | CONCEPTUS IMPLANTATION | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | EARLY-PREGNANCY | - |
dc.subject | PROTEIN-KINASE | - |
dc.subject | IMMUNE-SYSTEM | - |
dc.subject | PIG CONCEPTUS | - |
dc.title | Stimulatory effects of interleukin-1 beta on development of porcine uterine epithelial cell are mediated by activation of the ERK1/2 MAPK cell signaling cascade | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Gwonhwa | - |
dc.identifier.doi | 10.1016/j.mce.2015.10.022 | - |
dc.identifier.scopusid | 2-s2.0-84949603809 | - |
dc.identifier.wosid | 000370991700022 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR ENDOCRINOLOGY, v.419, no.C, pp.225 - 234 | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR ENDOCRINOLOGY | - |
dc.citation.title | MOLECULAR AND CELLULAR ENDOCRINOLOGY | - |
dc.citation.volume | 419 | - |
dc.citation.number | C | - |
dc.citation.startPage | 225 | - |
dc.citation.endPage | 234 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.subject.keywordPlus | FACTOR-KAPPA-B | - |
dc.subject.keywordPlus | PREIMPLANTATION MOUSE EMBRYOS | - |
dc.subject.keywordPlus | ENDOMETRIAL GLANDS | - |
dc.subject.keywordPlus | CONCEPTUS IMPLANTATION | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | EARLY-PREGNANCY | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | IMMUNE-SYSTEM | - |
dc.subject.keywordPlus | PIG CONCEPTUS | - |
dc.subject.keywordAuthor | Pig | - |
dc.subject.keywordAuthor | IL-1 beta | - |
dc.subject.keywordAuthor | Ped-implantation | - |
dc.subject.keywordAuthor | Uterine luminal epithelium | - |
dc.subject.keywordAuthor | ERK1/2 MAPK | - |
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