Analysis of microRNAs in a knock-in hESC line expressing epitope-tagged AGO2
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Young-Soo | - |
dc.contributor.author | Song, Hoseok | - |
dc.date.accessioned | 2021-09-04T04:14:25Z | - |
dc.date.available | 2021-09-04T04:14:25Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-01-02 | - |
dc.identifier.issn | 1976-8354 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/89851 | - |
dc.description.abstract | Argonaute (AGO) family proteins, which are key components of RNA-induced silencing complexes (RISCs), associate with both microRNAs (miRNAs) and mRNAs to regulate gene expression at post-transcriptional stages. While high-quality antibodies for AGO proteins are extremely useful for studies on the functions of miRNAs and AGO proteins, they are not always readily available. In this study, we generated a knock-in human embryonic stem cell (hESC) line by using homologous recombination to express FLAG-tagged AGO2 from its native genomic locus. FLAG-tagged AGO2 was exploited to analyze AGO2-bound miRNAs in hESCs cultured on a feeder layer by immunoprecipitation. Furthermore, the pluripotency of ESCs allowed for the analysis of miRNAs after differentiation into fibroblasts and cardiomyocytes. The genetically modified hESC line generated in this study will be highly useful for studying the functions of miRNA-mediated regulation in human development and cell differentiation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.subject | EMBRYONIC STEM-CELLS | - |
dc.subject | DEEP SEQUENCING DATA | - |
dc.subject | IDENTIFICATION | - |
dc.subject | GENOMICS | - |
dc.subject | CLEAVAGE | - |
dc.subject | MOUSE | - |
dc.subject | RNAI | - |
dc.subject | TOOL | - |
dc.title | Analysis of microRNAs in a knock-in hESC line expressing epitope-tagged AGO2 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Hoseok | - |
dc.identifier.doi | 10.1080/19768354.2015.1137227 | - |
dc.identifier.scopusid | 2-s2.0-84959156962 | - |
dc.identifier.wosid | 000371742900004 | - |
dc.identifier.bibliographicCitation | ANIMAL CELLS AND SYSTEMS, v.20, no.1, pp.24 - 30 | - |
dc.relation.isPartOf | ANIMAL CELLS AND SYSTEMS | - |
dc.citation.title | ANIMAL CELLS AND SYSTEMS | - |
dc.citation.volume | 20 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 24 | - |
dc.citation.endPage | 30 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002080222 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | DEEP SEQUENCING DATA | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | GENOMICS | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | RNAI | - |
dc.subject.keywordPlus | TOOL | - |
dc.subject.keywordAuthor | hESCs | - |
dc.subject.keywordAuthor | AGO2 | - |
dc.subject.keywordAuthor | microRNA | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.