An isolated Amycolatopsis sp GDS for cellulase and xylanase production using agricultural waste biomass
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kshirsagar, S. D. | - |
dc.contributor.author | Saratale, G. D. | - |
dc.contributor.author | Saratale, R. G. | - |
dc.contributor.author | Govindwar, S. P. | - |
dc.contributor.author | Oh, M. K. | - |
dc.date.accessioned | 2021-09-04T04:25:14Z | - |
dc.date.available | 2021-09-04T04:25:14Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-01 | - |
dc.identifier.issn | 1364-5072 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/89911 | - |
dc.description.abstract | Aim: The aim of this study was to evaluate an isolate of Amycolatopsis sp. GDS for cellulase and xylanase production, their characterization, and its application to the preparation of biomass feedstock for ethanol production. Methods and Results: A novel potent cellulolytic bacterial strain was isolated and identified as Amycolatopsis sp. GDS. The strain secreted high levels of cellulase and xylanase in the presence of agricultural waste biomass. The enzymes were thermostable and active up to 70 degrees C. Interestingly, the enzymes were expressed well at higher NaCl (up to 2.5 mol l(-1)) and ionic liquid (10%) concentrations, so that they could be used during the pretreatment of biomass. Enzyme stability in the presence of organic solvents, surfactants and oxidizing agents was also noted. Crude enzymes from Amycolatopsis sp. GDS resulted in comparable saccharification (60%) of wheat straw to commercial enzymes (64%). Conclusions: The cellulolytic enzymes from Amycolatopsis sp. GDS were stable, expressed well under conditions with various chemicals, and yielded significant amounts of hydrolysates from the biomass. The high bioethanol production using yeast co-cultures with enzymatic hydrolysates highlights the significance of selecting the strain and substrate for biofuel production. Significance and Impact of the Study: This study demonstrates the importance of the isolate Amycolatopsis sp. GDS that secretes high levels of cellulase and hemicellulase by utilizing agricultural waste biomass and its application in the preparation of biomass feedstock and sequential ethanol fermentation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | ENZYMATIC-HYDROLYSIS | - |
dc.subject | CELLULOLYTIC ENZYMES | - |
dc.subject | ETHANOL-PRODUCTION | - |
dc.subject | STREPTOMYCES SP | - |
dc.subject | LIGNOCELLULOSIC BIOMASS | - |
dc.subject | BIO-ETHANOL | - |
dc.subject | SACCHARIFICATION | - |
dc.subject | FERMENTATION | - |
dc.subject | OPTIMIZATION | - |
dc.subject | XYLOSE | - |
dc.title | An isolated Amycolatopsis sp GDS for cellulase and xylanase production using agricultural waste biomass | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, M. K. | - |
dc.identifier.doi | 10.1111/jam.12988 | - |
dc.identifier.scopusid | 2-s2.0-84955170706 | - |
dc.identifier.wosid | 000367686900011 | - |
dc.identifier.bibliographicCitation | JOURNAL OF APPLIED MICROBIOLOGY, v.120, no.1, pp.112 - 125 | - |
dc.relation.isPartOf | JOURNAL OF APPLIED MICROBIOLOGY | - |
dc.citation.title | JOURNAL OF APPLIED MICROBIOLOGY | - |
dc.citation.volume | 120 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 112 | - |
dc.citation.endPage | 125 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ENZYMATIC-HYDROLYSIS | - |
dc.subject.keywordPlus | CELLULOLYTIC ENZYMES | - |
dc.subject.keywordPlus | ETHANOL-PRODUCTION | - |
dc.subject.keywordPlus | STREPTOMYCES SP | - |
dc.subject.keywordPlus | LIGNOCELLULOSIC BIOMASS | - |
dc.subject.keywordPlus | BIO-ETHANOL | - |
dc.subject.keywordPlus | SACCHARIFICATION | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | XYLOSE | - |
dc.subject.keywordAuthor | Amycolatopsis sp GDS | - |
dc.subject.keywordAuthor | cellulase and xylanase | - |
dc.subject.keywordAuthor | enzymatic hydrolysis | - |
dc.subject.keywordAuthor | ethanol fermentation | - |
dc.subject.keywordAuthor | halotolerance | - |
dc.subject.keywordAuthor | wheat straw | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.