Determination of Manidipine in Human Plasma by UPLC-MS/MS and its Application to a Bioequivalence Study
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nguyen Lan Huong | - |
dc.contributor.author | Nguyen Huu Hoang | - |
dc.contributor.author | Hong, Sung-Yong | - |
dc.contributor.author | Park, Je Won | - |
dc.date.accessioned | 2021-09-04T05:04:30Z | - |
dc.date.available | 2021-09-04T05:04:30Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1573-4129 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/90176 | - |
dc.description.abstract | A rapid and highly sensitive method for the quantification of manidipine in human plasma was developed. Cleanup utilizing a 96-well solid-phase extraction technique is a simple and high-throughput method for extracting both manidipine and its internal standard felodipine from the quality-control samples: 97.5 +/- 2.1% for manidipine and 95.6 +/- 2.5% for IS. The ultra-performance liquid chromatography (UPLC) with tandem mass spectrometry (MS/MS) analytical tool, operated in a selective reaction monitoring mode was validated for manidipine quantification. The limit of detection and the lower limit of quantification were found to be 0.03 and 0.07 ng/mL, respectively. The intra-and inter-day precision was <3%, and accuracies ranged from 93.6 to 98.3%. A combination of the high-throughput SPE cleanup, and the sensitive and robust UPLC-MS/MS detection of manidipine at the picogram level was successfully applied to a bioequivalence study; T-max, C-max, and AUC(0-12) obtained from reference tablet were averaged at 2.26 h, 5.78 ng/mL, and 20.82 ng h/mL, whereas those from test one were at 2.31 h, 5.64 ng/mL, and 21.37 ng h/mL, respectively. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BENTHAM SCIENCE PUBL LTD | - |
dc.subject | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject | TANDEM MASS-SPECTROMETRY | - |
dc.subject | HUMAN-SERUM | - |
dc.subject | HYPERTENSION | - |
dc.title | Determination of Manidipine in Human Plasma by UPLC-MS/MS and its Application to a Bioequivalence Study | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Je Won | - |
dc.identifier.doi | 10.2174/1573412911999151012151244 | - |
dc.identifier.scopusid | 2-s2.0-84961575919 | - |
dc.identifier.wosid | 000374885700010 | - |
dc.identifier.bibliographicCitation | CURRENT PHARMACEUTICAL ANALYSIS, v.12, no.2, pp.152 - 156 | - |
dc.relation.isPartOf | CURRENT PHARMACEUTICAL ANALYSIS | - |
dc.citation.title | CURRENT PHARMACEUTICAL ANALYSIS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 152 | - |
dc.citation.endPage | 156 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | TANDEM MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | HUMAN-SERUM | - |
dc.subject.keywordPlus | HYPERTENSION | - |
dc.subject.keywordAuthor | Manidipine | - |
dc.subject.keywordAuthor | 96-well solid-phase extraction plate | - |
dc.subject.keywordAuthor | liquid chromatography | - |
dc.subject.keywordAuthor | mass spectrometry | - |
dc.subject.keywordAuthor | bioequivalence study | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.