Impaired angiogenesis in the enalapril-treated neonatal rat kidney
DC Field | Value | Language |
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dc.contributor.author | Yim, H.E. | - |
dc.contributor.author | Yoo, K.H. | - |
dc.contributor.author | Bae, E.S. | - |
dc.contributor.author | Hong, Y.S. | - |
dc.contributor.author | Lee, J.W. | - |
dc.date.accessioned | 2021-09-04T08:52:27Z | - |
dc.date.available | 2021-09-04T08:52:27Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1738-1061 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/91397 | - |
dc.description.abstract | Purpose: Nephrogenesis is normally accompanied by a tightly regulated and efficient vascularization. We investigated the effect of angiotensin II inhibition on angiogenesis in the developing rat kidney. Methods: Newborn rat pups were treated with enalapril (30 mg/kg/day) or vehicle (control) for 7 days after birth. Renal histological changes were checked using Hematoxylin & Eosin staining. We also investigated the intrarenal expression of vascular endothelial growth factor (VEGF)-A, VEGF receptor 1 (VEGFR1), VEGFR2, platelet-derived growth factor (PDGF)-B, and PDGF receptor-β with Western blotting and immunohistochemical staining at postnatal day 8. Expression of the endothelial cell marker CD31 was examined to determine glomerular and peritubular capillary density. Results: Enalapril-treated rat kidneys showed disrupted tubules and vessels when compared with the control rat kidneys. In the enalapril-treated group, intrarenal VEGF-A protein expression was significantly higher, whereas VEGFR1 protein expression was lower than that in the control group (P<0.05). The expression of VEGFR2, PDGF-B, and PDGF receptor-β was not different between the 2 groups. The increased capillary CD31 expression on the western blots of enalapril-treated rat kidneys indicated that the total endothelial cell protein level was increased, while the cortical capillary density, assessed using CD31 immunohistochemical staining, was decreased. Conclusion: Impaired VEGF-VEGFR signaling and altered capillary repair may play a role in the deterioration of the kidney vasculature after blocking of angiotensin II during renal development. © 2016 by The Korean Pediatric Society. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | Korean Pediatric Society | - |
dc.subject | angiotensin II | - |
dc.subject | beta actin | - |
dc.subject | CD31 antigen | - |
dc.subject | enalapril | - |
dc.subject | platelet derived growth factor B | - |
dc.subject | platelet derived growth factor beta receptor | - |
dc.subject | vasculotropin A | - |
dc.subject | vasculotropin receptor 1 | - |
dc.subject | vasculotropin receptor 2 | - |
dc.subject | angiogenesis | - |
dc.subject | animal experiment | - |
dc.subject | animal model | - |
dc.subject | animal tissue | - |
dc.subject | Article | - |
dc.subject | capillary density | - |
dc.subject | controlled study | - |
dc.subject | histology | - |
dc.subject | immunohistochemistry | - |
dc.subject | kidney development | - |
dc.subject | kidney vascularization | - |
dc.subject | newborn | - |
dc.subject | nonhuman | - |
dc.subject | protein expression | - |
dc.subject | rat | - |
dc.subject | vascularization | - |
dc.subject | Western blotting | - |
dc.title | Impaired angiogenesis in the enalapril-treated neonatal rat kidney | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yim, H.E. | - |
dc.contributor.affiliatedAuthor | Yoo, K.H. | - |
dc.contributor.affiliatedAuthor | Hong, Y.S. | - |
dc.identifier.doi | 10.3345/kjp.2016.59.1.8 | - |
dc.identifier.scopusid | 2-s2.0-84954560214 | - |
dc.identifier.bibliographicCitation | Korean Journal of Pediatrics, v.59, no.1, pp.8 - 15 | - |
dc.relation.isPartOf | Korean Journal of Pediatrics | - |
dc.citation.title | Korean Journal of Pediatrics | - |
dc.citation.volume | 59 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 8 | - |
dc.citation.endPage | 15 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordPlus | angiotensin II | - |
dc.subject.keywordPlus | beta actin | - |
dc.subject.keywordPlus | CD31 antigen | - |
dc.subject.keywordPlus | enalapril | - |
dc.subject.keywordPlus | platelet derived growth factor B | - |
dc.subject.keywordPlus | platelet derived growth factor beta receptor | - |
dc.subject.keywordPlus | vasculotropin A | - |
dc.subject.keywordPlus | vasculotropin receptor 1 | - |
dc.subject.keywordPlus | vasculotropin receptor 2 | - |
dc.subject.keywordPlus | angiogenesis | - |
dc.subject.keywordPlus | animal experiment | - |
dc.subject.keywordPlus | animal model | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | capillary density | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | histology | - |
dc.subject.keywordPlus | immunohistochemistry | - |
dc.subject.keywordPlus | kidney development | - |
dc.subject.keywordPlus | kidney vascularization | - |
dc.subject.keywordPlus | newborn | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | rat | - |
dc.subject.keywordPlus | vascularization | - |
dc.subject.keywordPlus | Western blotting | - |
dc.subject.keywordAuthor | Angiotensin II | - |
dc.subject.keywordAuthor | Growth and development | - |
dc.subject.keywordAuthor | Vascular remodeling | - |
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