Characterization and immunomodulation activity of Lactobacillus sakei L2 and L8 isolated from chicken cecum
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sim, I. | - |
dc.contributor.author | Park, K.-T. | - |
dc.contributor.author | Lim, Y.-H. | - |
dc.date.accessioned | 2021-09-04T08:53:13Z | - |
dc.date.available | 2021-09-04T08:53:13Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1598-642X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/91404 | - |
dc.description.abstract | The aim of this study was to investigate the potential of lactic acid bacteria (LAB) strains as probiotics. Two strains were isolated from healthy chicken cecum and their acid and bile tolerance, residual organic acids, antibacterial activity against pathogenic bacteria, and immunomodulation activity were measured. Identification of the isolated strains was performed using the API 50CHL system and phylogenetic analysis using 16S rDNA sequencing. The isolates were determined to be Lactobacillus sakei strains. The acid tolerance of strains L2 and L8 was high enough that 75% of the inoculum survived in pH 2 for 2 h. The bile tolerance of both strains was observed at a 1% Oxgall concentration in MRS broth. The production of organic acids (lactic acid and acetic acid) and pH changes during growth were monitored and the maximum concentrations were obtained after 48 h of incubation. Culture supernatants of the two LAB strains showed strong antibacterial activity against pathogenic bacteria. The heat-killed LAB cells also induced high levels of immune cell proliferation compared with the control, and stimulated IL-6 and TNF-α production in mouse macrophages. Therefore, L. sakei strains L2 and L8 can be considered suitable probiotic bacteria. © 2016, The Korean Society for Microbiology and Biotechnology. | - |
dc.language | Korean | - |
dc.language.iso | ko | - |
dc.publisher | Korean Society for Microbiolog and Biotechnology | - |
dc.subject | carboxylic acid | - |
dc.subject | DNA 16S | - |
dc.subject | interleukin 6 | - |
dc.subject | probiotic agent | - |
dc.subject | tumor necrosis factor alpha | - |
dc.subject | acetic acid | - |
dc.subject | interleukin 6 | - |
dc.subject | lactic acid | - |
dc.subject | probiotic agent | - |
dc.subject | tumor necrosis factor | - |
dc.subject | acid tolerance | - |
dc.subject | animal cell | - |
dc.subject | antibacterial activity | - |
dc.subject | Article | - |
dc.subject | bacterial growth | - |
dc.subject | bacterium identification | - |
dc.subject | cecum | - |
dc.subject | cell proliferation | - |
dc.subject | chicken | - |
dc.subject | concentration (parameters) | - |
dc.subject | cytokine production | - |
dc.subject | DNA sequence | - |
dc.subject | immunocompetent cell | - |
dc.subject | immunomodulation | - |
dc.subject | Lactobacillus sakei | - |
dc.subject | Lactobacillus sakei L2 | - |
dc.subject | Lactobacillus sakei L8 | - |
dc.subject | macrophage | - |
dc.subject | mouse | - |
dc.subject | nonhuman | - |
dc.subject | pH | - |
dc.subject | phylogeny | - |
dc.subject | antibacterial activity | - |
dc.subject | bacterium isolate | - |
dc.subject | chicken | - |
dc.subject | controlled study | - |
dc.subject | heat | - |
dc.title | Characterization and immunomodulation activity of Lactobacillus sakei L2 and L8 isolated from chicken cecum | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Y.-H. | - |
dc.identifier.doi | 10.4014/mbl.1512.12011 | - |
dc.identifier.scopusid | 2-s2.0-84994403223 | - |
dc.identifier.bibliographicCitation | Microbiology and Biotechnology Letters, v.44, no.2, pp.201 - 207 | - |
dc.relation.isPartOf | Microbiology and Biotechnology Letters | - |
dc.citation.title | Microbiology and Biotechnology Letters | - |
dc.citation.volume | 44 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 201 | - |
dc.citation.endPage | 207 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002116546 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordPlus | carboxylic acid | - |
dc.subject.keywordPlus | DNA 16S | - |
dc.subject.keywordPlus | interleukin 6 | - |
dc.subject.keywordPlus | probiotic agent | - |
dc.subject.keywordPlus | tumor necrosis factor alpha | - |
dc.subject.keywordPlus | acetic acid | - |
dc.subject.keywordPlus | interleukin 6 | - |
dc.subject.keywordPlus | lactic acid | - |
dc.subject.keywordPlus | probiotic agent | - |
dc.subject.keywordPlus | tumor necrosis factor | - |
dc.subject.keywordPlus | acid tolerance | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | antibacterial activity | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | bacterial growth | - |
dc.subject.keywordPlus | bacterium identification | - |
dc.subject.keywordPlus | cecum | - |
dc.subject.keywordPlus | cell proliferation | - |
dc.subject.keywordPlus | chicken | - |
dc.subject.keywordPlus | concentration (parameters) | - |
dc.subject.keywordPlus | cytokine production | - |
dc.subject.keywordPlus | DNA sequence | - |
dc.subject.keywordPlus | immunocompetent cell | - |
dc.subject.keywordPlus | immunomodulation | - |
dc.subject.keywordPlus | Lactobacillus sakei | - |
dc.subject.keywordPlus | Lactobacillus sakei L2 | - |
dc.subject.keywordPlus | Lactobacillus sakei L8 | - |
dc.subject.keywordPlus | macrophage | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | pH | - |
dc.subject.keywordPlus | phylogeny | - |
dc.subject.keywordPlus | antibacterial activity | - |
dc.subject.keywordPlus | bacterium isolate | - |
dc.subject.keywordPlus | chicken | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | heat | - |
dc.subject.keywordAuthor | Antibacterial activity | - |
dc.subject.keywordAuthor | Immunomodulation activity | - |
dc.subject.keywordAuthor | Lactobacillus sakei | - |
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