5-Aminolevulinic acid production in engineered Corynebacterium glutamicum via C-5 biosynthesis pathway
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ramzi, Ahmad Bazli | - |
dc.contributor.author | Hyeon, Jeong Eun | - |
dc.contributor.author | Kim, Seung Wook | - |
dc.contributor.author | Park, Chulhwan | - |
dc.contributor.author | Han, Sung Ok | - |
dc.date.accessioned | 2021-09-04T09:55:06Z | - |
dc.date.available | 2021-09-04T09:55:06Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/91667 | - |
dc.description.abstract | ALA (5-aminolevulinic acid) is an important intermediate in the synthesis of tetrapyrroles and the use of ALA has been gradually increasing in many fields, including medicine and agriculture. In this study, improved biological production of ALA in Corynebacterium glutamicum was achieved by overexpressing glutamate-initiated C-5 pathway. For this purpose, copies of the glutamyl t-RNA reductase HemA from several bacteria were mutated by site-directed mutagenesis of which a HemA version from Salmonella typhimurium exhibited the highest ALA production. Cultivation of the HemA-expressing strain produced approximately 204 mg/L of ALA, while co-expression with HemL (glutamate-1-semialdehyde aminotransferase) increased ALA concentration to 457 mg/L, representing 11.6- and 25.9-fold increases over the control strain (17 mg/L of ALA). Further effects of metabolic perturbation were investigated, leading to penicillin addition that further improves ALA production to 584 mg/L. In an optimized flask fermentation, engineered C. glutamicum strains expressing the HemA and hemAL operon produced up to 1.1 and 2.2 g/L ALA, respectively, under glutamate-producing conditions. The final yields represent 10.7- and 22.0-fold increases over the control strain (0.1 g/L of ALA). From these findings, ALA biosynthesis from glucose was successfully demonstrated and this study is the first to report ALA overproduction in C glutamicum via metabolic engineering. (C) 2015 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | EXPRESSING GLUTAMATE-DECARBOXYLASE | - |
dc.subject | TRANSFER-RNA REDUCTASE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | HEME-BIOSYNTHESIS | - |
dc.subject | AMINOLEVULINIC-ACID | - |
dc.subject | ENZYME-ACTIVITIES | - |
dc.subject | MICROORGANISMS | - |
dc.subject | DEHYDROGENASE | - |
dc.subject | INCREASES | - |
dc.subject | GENE | - |
dc.title | 5-Aminolevulinic acid production in engineered Corynebacterium glutamicum via C-5 biosynthesis pathway | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Wook | - |
dc.contributor.affiliatedAuthor | Han, Sung Ok | - |
dc.identifier.doi | 10.1016/j.enzmictec.2015.07.004 | - |
dc.identifier.scopusid | 2-s2.0-84939824310 | - |
dc.identifier.wosid | 000363353000001 | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.81, pp.1 - 7 | - |
dc.relation.isPartOf | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 81 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | EXPRESSING GLUTAMATE-DECARBOXYLASE | - |
dc.subject.keywordPlus | TRANSFER-RNA REDUCTASE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | HEME-BIOSYNTHESIS | - |
dc.subject.keywordPlus | AMINOLEVULINIC-ACID | - |
dc.subject.keywordPlus | ENZYME-ACTIVITIES | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
dc.subject.keywordPlus | DEHYDROGENASE | - |
dc.subject.keywordPlus | INCREASES | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | 5-Aminolevulinic acid | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | C-5 pathway | - |
dc.subject.keywordAuthor | Glutamyl t-RNA reductase | - |
dc.subject.keywordAuthor | Glutamate | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.