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Enhanced Cytoplasmic Delivery of RAGE siRNA Using Bioreducible Polyethylenimine-based Nanocarriers for Myocardial Gene Therapy
- Authors
- Yang, Min Jung; Ku, Sook Hee; Kim, Dongkyu; Kim, Won Jong; Mok, Hyejung; Kim, Sun Hwa; Kwon, Ick Chan
- Issue Date
- 12월-2015
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- bioreducible polyethylenimine; cytoplasmic delivery; myocardial infarction; RAGE; siRNA
- Citation
- MACROMOLECULAR BIOSCIENCE, v.15, no.12, pp.1755 - 1763
- Indexed
- SCIE
SCOPUS
- Journal Title
- MACROMOLECULAR BIOSCIENCE
- Volume
- 15
- Number
- 12
- Start Page
- 1755
- End Page
- 1763
- ISSN
- 1616-5187
- Abstract
- This study aims to develop bioreducible polyethylenimine (rPEI)/siRNA polyplexes with high stability, high transfection efficiency, and low cytotoxicity for efficient cytoplasmic siRNA delivery. rPEI successfully incorporated siRNA into stable and compact nano-complexes, and the disulfide linkages in rPEI/siRNA were cleaved under reductive environments, resulting in efficient intracellular translocation and siRNA release. In this study, receptor for advanced glycation end-products (RAGE) was selected as a therapeutic target gene because it is associated with inflammatory responses in ischemia/reperfusion injury. rPEI/siRAGE exhibited high target gene silencing and low cytotoxicity in cardiomyocytes, and the treatment of rPEI/siRAGE reduced the myocardial infarction size.
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