Label-free detection of ApoE4-mediated beta-amyloid aggregation on single nanoparticle uncovering Alzheimer's disease
DC Field | Value | Language |
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dc.contributor.author | Kang, Min Kyung | - |
dc.contributor.author | Lee, Jeewon | - |
dc.contributor.author | Nguyen, Anh H. | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.date.accessioned | 2021-09-04T11:27:04Z | - |
dc.date.available | 2021-09-04T11:27:04Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2015-10-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92180 | - |
dc.description.abstract | Beta amyloid (A beta) deposition is a pathological milestone of Alzheimer's disease (AD). This is facilitated by an isoform of Apolipoprotein E4 (ApoE4), which is a dominant risk factor for AD. However, current in vitro A beta aggregation assays were performed in extreme conditions not linked to physiological conditions, to understand the mechanism of A beta induced neurotoxicity. Here, we present a simple method for the ApoE4-mediated A beta aggregation at physiological conditions using single gold nanoparticle based on localized surface plasmon resonance (LSPR). It can be directly observed by dark-field microscope or even by the naked eye. Following LSPR principles, we used ApoE4 inducing A beta 42 self-assemblies on gold nanoparticles (AuNPs) surface via their surface charge interaction. Using physiologically mimic cerebrospinal fluid, we determined a detection limit of 1.5 pM for A beta 42 corresponding to the similar to 2.9 nm LSPR-peak shift under ApoE4. Interestingly, the result also shows that ApoE4 induces the aggregation of A beta 42 more specifically and rapidly than that of A beta 40. This is the first biomimetic platform for real-time detection of A beta aggregation, mimicking biological conditions, which can be used to investigate AD directly. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | APOLIPOPROTEIN-E GENOTYPE | - |
dc.subject | A-BETA | - |
dc.subject | QUANTITATIVE-ANALYSIS | - |
dc.subject | MOUSE MODEL | - |
dc.subject | PEPTIDE | - |
dc.subject | APOE | - |
dc.subject | DEPOSITION | - |
dc.subject | GOLD | - |
dc.subject | A-BETA-42 | - |
dc.subject | BINDING | - |
dc.title | Label-free detection of ApoE4-mediated beta-amyloid aggregation on single nanoparticle uncovering Alzheimer's disease | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jeewon | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1016/j.bios.2015.05.017 | - |
dc.identifier.scopusid | 2-s2.0-84929306979 | - |
dc.identifier.wosid | 000356734000029 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.72, pp.197 - 204 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 72 | - |
dc.citation.startPage | 197 | - |
dc.citation.endPage | 204 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | APOLIPOPROTEIN-E GENOTYPE | - |
dc.subject.keywordPlus | A-BETA | - |
dc.subject.keywordPlus | QUANTITATIVE-ANALYSIS | - |
dc.subject.keywordPlus | MOUSE MODEL | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | APOE | - |
dc.subject.keywordPlus | DEPOSITION | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | A-BETA-42 | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordAuthor | Gold nanoparticles (AuNPs) | - |
dc.subject.keywordAuthor | Localized surface plasmon resonance (LSPR) | - |
dc.subject.keywordAuthor | Rayleigh scattering | - |
dc.subject.keywordAuthor | Beta amyloid (A beta) | - |
dc.subject.keywordAuthor | Apolipoprotein4 (ApoE4) | - |
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