The effects of human recombinant granulocyte-colony stimulating factor treatment during in vitro maturation of porcine oocyte on subsequent embryonic development
DC Field | Value | Language |
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dc.contributor.author | Cai, Lian | - |
dc.contributor.author | Jeon, Yubyeol | - |
dc.contributor.author | Yoon, Junchul David | - |
dc.contributor.author | Hwang, Seon-Ung | - |
dc.contributor.author | Kim, Eunhye | - |
dc.contributor.author | Park, Kyu-mi | - |
dc.contributor.author | Kim, Kyu-Jun | - |
dc.contributor.author | Jin, Ming Hui | - |
dc.contributor.author | Lee, Eunsong | - |
dc.contributor.author | Kim, Hyunggee | - |
dc.contributor.author | Jeung, Eui-bae | - |
dc.contributor.author | Hyun, Sang Hwan | - |
dc.date.accessioned | 2021-09-04T11:28:21Z | - |
dc.date.available | 2021-09-04T11:28:21Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2015-10-15 | - |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92189 | - |
dc.description.abstract | Granulocyte colony-stimulating factor (G-CSF) is required for proliferation, differentiation, and survival of cells. It is also a biomarker of human oocyte developmental competence for embryo implantation. In humans, the G-CSF concentration peaks during the ovulatory phase of the ovarian cycle. In this study, the expressions of G-CSF and its receptor were analyzed by polymerase chain reaction in granulosa cells (GCs), CL, cumulus cells (CCs), and oocytes. Cumulus-oocyte complexes were aspirated from antral follicles of 1 to 3 mm (small follicles) and 4 to 6 mm (medium follicles). Cumulus-oocyte complexes from two kinds of follicles were matured in protein-free maturation medium supplemented with various concentrations of G-CSF (0, 10, and 100 ng/mL). By real-time polymerase chain reaction, the expressions of G-CSF and its receptor were detected in GCs, CL CCs, and oocytes. Interestingly, the G-CSF transcript levels were significantly lower in oocytes than in the other cell types, whereas the G-CSF receptor transcript levels in oocytes were similar to those in GCs. After 44 hours of IVM, no differences in the rate of nuclear maturation were detected; however, the intracellular reactive oxygen species levels in oocytes from both groups of follicles matured with 10 ng/mL of human recombinant G-CSF (hrG-CSF) groups were significantly lower (P < 0.05). After parthenogenetic activation, the cleavage rates were significantly (P < 0.05) higher in 100 ng/mL hrG-CSF-treated small (63.3%) follicles than in 0,10 ng/mL hrG-CSF-treated small (38.6% and 49.0%, respectively) follicles and 0 ng/mL hrG-CSF-treated medium (52.1%) follicles, and the cleavage rates were significantly (P < 0.05) higher in 10 ng/mL hrG-CSF-treated medium (763%) follicles than in all other groups. The blastocyst formation rates were significantly (P < 0.05) higher in 100 ng/mL hrG-CSF-treated small (31.2%) follicles than in 0 and 10 ng/mL hrG-CSF small (10.4% and 15.6%, respectively) follicles, and the 10 ng/mL hrG-CSF medium (45.7%) follicle was significantly (P < 0.05) higher than in all other groups. The total cell number in blastocysts from the 10 ng/mL hrG-CSF medium (106.5) follicles was significantly (P < 0.05) increased compared to 0, 10, 100 ng/mL hrG-CSF small (55.0, 73.7 and 59.5, respectively) follicles and 0, 100 ng/mL hrG-CSF-treated medium (82.5 and 93.5, respectively) follicles. After IVF, the blastocysts stage was significantly (P < 0.05) increased in 10 ng/mL hrG-CSF-treated medium (36.4%) follicles. Fertilization efficiency was significantly high in 100 ng/mL of small (29.1%) and 10 ng/mL of medium (44.0%) follicles. We also examined the Bcl2 and ERK2 transcript levels and found that they were significantly higher in the small and medium follicle treatment groups. In conclusion, these results indicate that hrG-CSF improve the viability of porcine embryos. (C) 2015 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | FACTOR G-CSF | - |
dc.subject | MALE PRONUCLEAR FORMATION | - |
dc.subject | CELL NUCLEAR TRANSFER | - |
dc.subject | FOLLICULAR G-CSF | - |
dc.subject | CUMULUS CELLS | - |
dc.subject | BOVINE OOCYTES | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | PREIMPLANTATION DEVELOPMENT | - |
dc.subject | PARTHENOGENETIC ACTIVATION | - |
dc.title | The effects of human recombinant granulocyte-colony stimulating factor treatment during in vitro maturation of porcine oocyte on subsequent embryonic development | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyunggee | - |
dc.identifier.doi | 10.1016/j.theriogenology.2015.06.008 | - |
dc.identifier.scopusid | 2-s2.0-84940604293 | - |
dc.identifier.wosid | 000361077700004 | - |
dc.identifier.bibliographicCitation | THERIOGENOLOGY, v.84, no.7, pp.1075 - 1087 | - |
dc.relation.isPartOf | THERIOGENOLOGY | - |
dc.citation.title | THERIOGENOLOGY | - |
dc.citation.volume | 84 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1075 | - |
dc.citation.endPage | 1087 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.subject.keywordPlus | FACTOR G-CSF | - |
dc.subject.keywordPlus | MALE PRONUCLEAR FORMATION | - |
dc.subject.keywordPlus | CELL NUCLEAR TRANSFER | - |
dc.subject.keywordPlus | FOLLICULAR G-CSF | - |
dc.subject.keywordPlus | CUMULUS CELLS | - |
dc.subject.keywordPlus | BOVINE OOCYTES | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | PREIMPLANTATION DEVELOPMENT | - |
dc.subject.keywordPlus | PARTHENOGENETIC ACTIVATION | - |
dc.subject.keywordAuthor | Porcine | - |
dc.subject.keywordAuthor | Oocyte | - |
dc.subject.keywordAuthor | IVM | - |
dc.subject.keywordAuthor | Granulocyte colony-stimulating factor | - |
dc.subject.keywordAuthor | Follicle size | - |
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