Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering
DC Field | Value | Language |
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dc.contributor.author | Kim, Sung Eun | - |
dc.contributor.author | Yun, Young-Pil | - |
dc.contributor.author | Shim, Kyu-Sik | - |
dc.contributor.author | Park, Kyeongsoon | - |
dc.contributor.author | Choi, Sung-Wook | - |
dc.contributor.author | Shin, Dong Hyup | - |
dc.contributor.author | Suh, Dong Hun | - |
dc.date.accessioned | 2021-09-04T11:47:34Z | - |
dc.date.available | 2021-09-04T11:47:34Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2015-10-01 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92228 | - |
dc.description.abstract | The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | MESENCHYMAL STEM-CELLS | - |
dc.subject | MORPHOGENIC PROTEIN-2 | - |
dc.subject | IN-VITRO | - |
dc.subject | POLY(LACTIC-CO-GLYCOLIC ACID) | - |
dc.subject | OSTEOGENIC DIFFERENTIATION | - |
dc.subject | CONTROLLED-RELEASE | - |
dc.subject | PLGA MICROSPHERES | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | SCAFFOLDS | - |
dc.subject | TITANIUM | - |
dc.title | Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, Dong Hun | - |
dc.identifier.doi | 10.1016/j.colsurfb.2015.05.003 | - |
dc.identifier.scopusid | 2-s2.0-84938071385 | - |
dc.identifier.wosid | 000362142000057 | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, v.134, pp.453 - 460 | - |
dc.relation.isPartOf | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.title | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.volume | 134 | - |
dc.citation.startPage | 453 | - |
dc.citation.endPage | 460 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | MORPHOGENIC PROTEIN-2 | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | POLY(LACTIC-CO-GLYCOLIC ACID) | - |
dc.subject.keywordPlus | OSTEOGENIC DIFFERENTIATION | - |
dc.subject.keywordPlus | CONTROLLED-RELEASE | - |
dc.subject.keywordPlus | PLGA MICROSPHERES | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | TITANIUM | - |
dc.subject.keywordAuthor | Bone morphogenic protein-2 (BMP-2) | - |
dc.subject.keywordAuthor | Porous microspheres | - |
dc.subject.keywordAuthor | Fluidic device | - |
dc.subject.keywordAuthor | Heparin | - |
dc.subject.keywordAuthor | Bone tissue engineering | - |
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