Timescale analysis for estimating upper limit perfusion rate in a microfluidic perfusion cell culture platform
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Maeng, Joon-Ho | - |
dc.contributor.author | Jeong, Hyo Eun | - |
dc.contributor.author | Shin, Hyun-Joon | - |
dc.contributor.author | Kim, Sehoon | - |
dc.contributor.author | Lee, Jong-Chul | - |
dc.contributor.author | Lee, Jaeyoung | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Lee, Sangyoup | - |
dc.date.accessioned | 2021-09-04T11:56:45Z | - |
dc.date.available | 2021-09-04T11:56:45Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 1613-4982 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92293 | - |
dc.description.abstract | Proliferation characteristics of CHO-K1 cells were investigated under a variety of medium perfusion rate conditions in a microfluidic perfusion cell culture platform. Five microcavities of were adopted in order to minimize or isolate the shear effects on cell surfaces. Microchannels of serially connecting these microcavities created flow contractions and expansions repeatedly, resulting in two different diffusion and convection timescales through the platform. Average shear stresses on the bottom of microcavity were both numerically and analytically estimated, and medium flow was operated at rates where shear stress is below 2 mPa. Proliferation rates of CHO-K1 cells were investigated based both on population groups derived from the number of initially seeded cells and on the microcavity locations. Population groups showed minimal influences on proliferation rates, while proliferation rates increased clearly with medium perfusion rates. Strong effects of microcavity locations were observed on proliferation at . Such effects were analyzed by investigating the relationships of reaction, diffusion, and convection timescales associated with perfusion conditions. The ratio of diffusion timescale and convection timescale was suggested as a guideline to estimate the upper limit of perfusion rate in microfluidic perfusion cell culture platform. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.subject | SHEAR-STRESS | - |
dc.subject | SEEDING DENSITY | - |
dc.subject | GROWTH | - |
dc.subject | DEVICE | - |
dc.title | Timescale analysis for estimating upper limit perfusion rate in a microfluidic perfusion cell culture platform | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Seok | - |
dc.identifier.doi | 10.1007/s10404-015-1602-4 | - |
dc.identifier.scopusid | 2-s2.0-84942982343 | - |
dc.identifier.wosid | 000361986400002 | - |
dc.identifier.bibliographicCitation | MICROFLUIDICS AND NANOFLUIDICS, v.19, no.4, pp.777 - 786 | - |
dc.relation.isPartOf | MICROFLUIDICS AND NANOFLUIDICS | - |
dc.citation.title | MICROFLUIDICS AND NANOFLUIDICS | - |
dc.citation.volume | 19 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 777 | - |
dc.citation.endPage | 786 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Physics, Fluids & Plasmas | - |
dc.subject.keywordPlus | SHEAR-STRESS | - |
dc.subject.keywordPlus | SEEDING DENSITY | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | DEVICE | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.