A New Shuttle Plasmid That Stably Replicates in Clostridium acetobutylicum
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sang-Hyun | - |
dc.contributor.author | Kwon, Min-A | - |
dc.contributor.author | Choi, Sunhwa | - |
dc.contributor.author | Kim, Sooah | - |
dc.contributor.author | Kim, Jungyeon | - |
dc.contributor.author | Shin, Yong-An | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.date.accessioned | 2021-09-04T12:02:37Z | - |
dc.date.available | 2021-09-04T12:02:37Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92340 | - |
dc.description.abstract | We have developed a new shuttle plasmid, designated as pLK1-MCS that can replicate in both Clostridium acetobutylicum and Escherichia coli, by combining the pUB110 and pUC19 plasmids. Plasmid pLK1-MCS replicated more stably than previously reported plasmids containing either the pIM13 or the pAMa1 replicon in the absence of antibiotic selective pressure. The transfer frequency of pLK1-MCS into C. acetobutylicum was similar to the transfer frequency of other shuttle plasmids. We complemented C. acetobutylicum ML1 (that does not produce solvents such as acetone, butanol, and ethanol owing to loss of the megaplasmid pSOL1 harboring the adhE1-ctfAB-adc operon) by introducing pLK1-MCS carrying the adhE1-ctfAB-adc operon into C. acetobutylicum ML1. The transformed cells were able to resume anaerobic solvent production, indicating that the new shuttle plasmid has the potential for practical use in microbial biotechnology. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | BACILLUS-SUBTILIS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | GENOME SEQUENCE | - |
dc.subject | BUTANOL | - |
dc.subject | TRANSFORMATION | - |
dc.subject | CLONING | - |
dc.subject | FERMENTATION | - |
dc.subject | RESTRICTION | - |
dc.subject | EXPRESSION | - |
dc.subject | GENES | - |
dc.title | A New Shuttle Plasmid That Stably Replicates in Clostridium acetobutylicum | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kyoung Heon | - |
dc.identifier.doi | 10.4014/jmb.1504.04070 | - |
dc.identifier.scopusid | 2-s2.0-84945286565 | - |
dc.identifier.wosid | 000365443800017 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.10, pp.1702 - 1708 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 25 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1702 | - |
dc.citation.endPage | 1708 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002041691 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENOME SEQUENCE | - |
dc.subject.keywordPlus | BUTANOL | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | RESTRICTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordAuthor | Clostridium acetobutylicum | - |
dc.subject.keywordAuthor | shuttle plasmid | - |
dc.subject.keywordAuthor | segregational stability | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.