Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, In Jung | - |
dc.contributor.author | Nam, Ki Hyun | - |
dc.contributor.author | Yun, Eun Ju | - |
dc.contributor.author | Kim, Sooah | - |
dc.contributor.author | Youn, Hak Jin | - |
dc.contributor.author | Lee, Hee Jin | - |
dc.contributor.author | Choi, In-Geol | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.date.accessioned | 2021-09-04T12:13:32Z | - |
dc.date.available | 2021-09-04T12:13:32Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92418 | - |
dc.description.abstract | Auxiliary activity family 9 (AA9, formerly known as glycoside hydrolase family 61 or polysaccharide monooxygenase) is a group of fungal proteins that were recently found to have a significant synergism with cellulase in cellulose hydrolysis via the oxidative cleavage of glycosidic bonds of cellulose chains. In this study, we report the active expression of a recombinant fungal AA9 from Chaetomium globosum (CgAA9) in a bacterial host, Escherichia coli, and the optimization of its synergistic activity in cellulose hydrolysis by using cellulase. The recombinant CgAA9 (0.9 mg/g cellulose) exhibited 1.7-fold synergism in the hydrolysis of Avicel when incubated with 0.9 filter paper units of Celluclast 1.5 L/g cellulose. The first study of the active expression of AA9 using a bacterial host and its synergistic optimization could be useful for the industrial application of AA9 for the saccharification of lignocellulose. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | GLYCOSIDE HYDROLASE FAMILY | - |
dc.subject | ENZYMATIC-HYDROLYSIS | - |
dc.subject | POLYSACCHARIDE MONOOXYGENASES | - |
dc.subject | NEUROSPORA-CRASSA | - |
dc.subject | PHANEROCHAETE-CHRYSOSPORIUM | - |
dc.subject | CELLOBIOSE DEHYDROGENASE | - |
dc.subject | DEGRADATION | - |
dc.subject | ENZYMES | - |
dc.subject | BIOMASS | - |
dc.subject | CONVERSION | - |
dc.title | Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, In Jung | - |
dc.contributor.affiliatedAuthor | Choi, In-Geol | - |
dc.contributor.affiliatedAuthor | Kim, Kyoung Heon | - |
dc.identifier.doi | 10.1007/s00253-015-6592-3 | - |
dc.identifier.scopusid | 2-s2.0-84941996921 | - |
dc.identifier.wosid | 000361493200019 | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.99, no.20, pp.8537 - 8547 | - |
dc.relation.isPartOf | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 99 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 8537 | - |
dc.citation.endPage | 8547 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | GLYCOSIDE HYDROLASE FAMILY | - |
dc.subject.keywordPlus | ENZYMATIC-HYDROLYSIS | - |
dc.subject.keywordPlus | POLYSACCHARIDE MONOOXYGENASES | - |
dc.subject.keywordPlus | NEUROSPORA-CRASSA | - |
dc.subject.keywordPlus | PHANEROCHAETE-CHRYSOSPORIUM | - |
dc.subject.keywordPlus | CELLOBIOSE DEHYDROGENASE | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | BIOMASS | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordAuthor | AA9 | - |
dc.subject.keywordAuthor | GH61 | - |
dc.subject.keywordAuthor | Synergism | - |
dc.subject.keywordAuthor | Cellulose hydrolysis | - |
dc.subject.keywordAuthor | Cellulase | - |
dc.subject.keywordAuthor | Chaetomium globosum | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.