Characterization of Phosphoenolpyruvate Carboxylase from Oceanimonas smirnovii in Escherichia coli
DC Field | Value | Language |
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dc.contributor.author | Park, Soohyun | - |
dc.contributor.author | Lee, Wangjun | - |
dc.contributor.author | Kim, Hyeonsoo | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.contributor.author | Lee, Jinwon | - |
dc.date.accessioned | 2021-09-04T13:10:52Z | - |
dc.date.available | 2021-09-04T13:10:52Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-09 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92654 | - |
dc.description.abstract | In this study, phosphoenolpyruvate carboxylase (PEPC) derived from Oceanimonas smirnovii (OS) was expressed as a soluble protein in Escherichia coli BL21(DE3). We isolated OS-PEPC (a recombinant PEPC protein) by his-tag purification. The purified protein showed a single band upon analysis with SDS-PAGE, and it had an apparent molecular mass of 98 kDa. Pufied OS-PEPC showed a specific activity value of 21.8 +/- A 0.495 U/mg protein. Especially, OS-PEPC showed the enzymatic activity between 40 and 50 A degrees C. It maintained enzymatic activity in basic pH conditions (pH value, 9-10). We also measured OS-PEPC PEP and HCO3 (-) saturation kinetics and confirmed the effect of divalent cation on OS-PEPC activity. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | HUMANA PRESS INC | - |
dc.subject | CARBONIC-ANHYDRASE | - |
dc.subject | EXPRESSION | - |
dc.subject | ENZYMES | - |
dc.subject | GENE | - |
dc.title | Characterization of Phosphoenolpyruvate Carboxylase from Oceanimonas smirnovii in Escherichia coli | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1007/s12010-015-1739-3 | - |
dc.identifier.scopusid | 2-s2.0-84940447103 | - |
dc.identifier.wosid | 000360075800016 | - |
dc.identifier.bibliographicCitation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.177, no.1, pp.217 - 225 | - |
dc.relation.isPartOf | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.volume | 177 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 217 | - |
dc.citation.endPage | 225 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | CARBONIC-ANHYDRASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | Oceanimonas smirnovii | - |
dc.subject.keywordAuthor | Phosphoenolpyruvate carboxylase | - |
dc.subject.keywordAuthor | Characterization | - |
dc.subject.keywordAuthor | Bicarbonate | - |
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