Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock
DC Field | Value | Language |
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dc.contributor.author | Saratale, Ganesh D. | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-04T13:14:24Z | - |
dc.date.available | 2021-09-04T13:14:24Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-09 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92676 | - |
dc.description.abstract | Alkaline pretreatment using NaOH, KOH, or NaOCl has been applied to various types of waste biomass to enhance enzymatic digestibility. Pretreatment (2% NaOH, 121 degrees C, 30 min) of rice paddy straw (PS) resulted in a maximum yield of 703 mg of reducing sugar per gram of PS with 84.19% hydrolysis yield after a two-step enzymatic hydrolysis process. Ralstonia eutropha ATCC 17699 was tested for its ability to synthesize poly-3-hydroxybutyrate (PHB) using PS hydrolysates as its sole carbon source. It is noteworthy that dry cell weight, polyhydroxyalkanoate (PHA) accumulation and PHB yield with the use of laboratory-grade sugars were similar to those achieved with PS-derived sugars. Under optimized conditions, we observed maximal PHA accumulation (75.45%) and PHB production (11.42 g/L) within 48 h of fermentation. After PHB recovery, the physicochemical properties of PHB were determined by various analytical techniques, showed the results were consistent with the characteristics of a standard polymer of PHB. Thus, the PS hydrolysate proved to be an excellent cheap carbon substrate for PHB production. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | SOLID-STATE FERMENTATION | - |
dc.subject | BIOETHANOL PRODUCTION | - |
dc.subject | ENZYMATIC-HYDROLYSIS | - |
dc.subject | RICE STRAW | - |
dc.subject | LIGNOCELLULOSIC BIOMASS | - |
dc.subject | BIOHYDROGEN PRODUCTION | - |
dc.subject | CELLULOLYTIC ENZYMES | - |
dc.subject | CARBON SOURCE | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | TECHNOLOGIES | - |
dc.title | Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2015.07.034 | - |
dc.identifier.scopusid | 2-s2.0-84938067741 | - |
dc.identifier.wosid | 000361251000075 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.80, pp.627 - 635 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 80 | - |
dc.citation.startPage | 627 | - |
dc.citation.endPage | 635 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | SOLID-STATE FERMENTATION | - |
dc.subject.keywordPlus | BIOETHANOL PRODUCTION | - |
dc.subject.keywordPlus | ENZYMATIC-HYDROLYSIS | - |
dc.subject.keywordPlus | RICE STRAW | - |
dc.subject.keywordPlus | LIGNOCELLULOSIC BIOMASS | - |
dc.subject.keywordPlus | BIOHYDROGEN PRODUCTION | - |
dc.subject.keywordPlus | CELLULOLYTIC ENZYMES | - |
dc.subject.keywordPlus | CARBON SOURCE | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | TECHNOLOGIES | - |
dc.subject.keywordAuthor | Rice paddy straw | - |
dc.subject.keywordAuthor | Alkaline pretreatment | - |
dc.subject.keywordAuthor | Poly-3-hydroxybutyrate | - |
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