Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Fabrication of plasmon length-based surface enhanced Raman scattering for multiplex detection on microfluidic device

Authors
Nguyen, Anh H.Lee, JeewonChoi, Hong IlKwak, Ho SeokSim, Sang Jun
Issue Date
15-8월-2015
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
SERS; Multiplex detection; Microfluidics; Plasmonic nanoparticles; Cancer biomarkers
Citation
BIOSENSORS & BIOELECTRONICS, v.70, pp.358 - 365
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
70
Start Page
358
End Page
365
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/92748
DOI
10.1016/j.bios.2015.03.064
ISSN
0956-5663
Abstract
The length of bioreceptors plays an important role in signal enhancement of surface-enhanced Raman scattering (SERS) due to amplification of electromagnetic fields generated by the excitation of localized surface plasmons. Herein, intact antibodies (IgG) and Fab fragments conjugated onto gold nanostar were used to fabricate two kinds of immunosensors for measurement of their SERS signals. Using CA125 as the antigen and Rhodamine-6G (R6G)-conjugated immunogolds, a SERS immunosensor was self-assembled by antigen-antibody interaction. The results showed that the SERS signal from the Fab immunosensor was 2.4 times higher than that of the IgG immunosensor. Furthermore, increased hot-spots by silver atom deposition onto the IgG and Fab immunosensor showed 2.1 and 1.4 times higher signals than before enhancement, respectively. For application, based on the Fab immunosensor, a SERS-compatible microfluidic system was designed for multiplex assays to overcome the drawbacks of conventional assays. This system can measure biological specimens directly from bio fluids instead of using a complex microfluidic device containing separation and detection elements. Four approved biomarkers of breast cancer, including cancer antigen (CA125), HER2, epididymis protein (HE4), and Eotaxin-1, were detected from patient-mimicked serum with limits of 15 fM, 17 fM, 21 fM, and 6.5 fM, respectively. The results indicated that the lengths and geometry of the bioreceptors determined the intensity of SERS signal from the interface and cavity of the sandwich immunosensor. Silver atom deposition at the cavity of the immunosensor increased the SERS signal. Finally, the SERS immunosensor built-in microfluidic system improved the performance of multiplex diagnostics. (C) 2015 Elsevier B.V. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Sim, Sang Jun photo

Sim, Sang Jun
공과대학 (화공생명공학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE