AAM-B Interacts with Nonstructural 4B and Regulates Hepatitis C Virus Propagation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Eun-Mee | - |
dc.contributor.author | Lim, Yun-Sook | - |
dc.contributor.author | Ahn, Byung-Yoon | - |
dc.contributor.author | Hwang, Soon B. | - |
dc.date.accessioned | 2021-09-04T14:13:18Z | - |
dc.date.available | 2021-09-04T14:13:18Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2015-07-17 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/92999 | - |
dc.description.abstract | Hepatitis C virus (HCV) usurps host cellular lipid metabolism for production of infectious virus particles. Recently, we have screened a siRNA library targeting host factors that control lipid metabolism and lipid droplet (LD) formation in cell culture grown HCV (HCVcc)-infected cells. Of 10 final candidates, we selected the gene encoding AAM-B for further characterization. We showed that siRNA-mediated knockdown of AAM-B impaired HCV propagation in Jc1-infected cells. More precisely, knockdown of AAM-B abrogated production of infectious HCV particles in both Jc1 RNA electroporated cells and Jc1-infected cells. It is worth noting that knockdown of AAM-B exerted no effect on lipid droplet formation. Moreover, AAM-B interacted with nonstructural 4B (NS4B) through the C-terminal region of NS4B. Protein interplay between AAM-B and NS4B was verified in the context of HCV replication. Using either transient or stable expression of AAM-B, we verified that AAM-B colocalized with NS4B in the cytoplasm. Immunofluorescence data further showed that AAM-B might be involved in recruitment of NS4B to sites in close proximity to LDs to facilitate HCV propagation. Collectively, this study provides new insight into how HCV utilizes cellular AAM-B to facilitate viral propagation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | LIPID DROPLETS | - |
dc.subject | REPLICATION | - |
dc.subject | PROTEIN | - |
dc.subject | ORGANELLE | - |
dc.subject | GENOME | - |
dc.subject | TIP47 | - |
dc.title | AAM-B Interacts with Nonstructural 4B and Regulates Hepatitis C Virus Propagation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ahn, Byung-Yoon | - |
dc.identifier.doi | 10.1371/journal.pone.0132839 | - |
dc.identifier.scopusid | 2-s2.0-84940888301 | - |
dc.identifier.wosid | 000358198700065 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.10, no.7 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 10 | - |
dc.citation.number | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | LIPID DROPLETS | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ORGANELLE | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | TIP47 | - |
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