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Multiplex diagnosis of viral infectious diseases (AIDS, hepatitis C, and hepatitis A) based on point of care lateral flow assay using engineered proteinticles

Authors
Lee, Jong-HwanSeo, Hyuk SeongKwon, Jung-HyukKim, Hee-TaeKwon, Koo ChulSim, Sang JunCha, Young JooLee, Jeewon
Issue Date
15-7월-2015
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
Proteinticles; Surface engineering; Multiplex diagnosis; Lateral flow assay; Infectious diseases
Citation
BIOSENSORS & BIOELECTRONICS, v.69, pp.213 - 225
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
69
Start Page
213
End Page
225
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/93004
DOI
10.1016/j.bios.2015.02.033
ISSN
0956-5663
Abstract
Lateral flow assay (LFA) is an attractive method for rapid, simple, and cost-effective point of care diagnosis. For LFA-based multiplex diagnosis of three viral intractable diseases (acquired immune deficiency syndrome and hepatitis C and A), here we developed proteinticle-based 7 different 3D probes that display different viral antigens on their surface, which were synthesized in Escherichia coli by self-assembly of human ferritin heavy chain that was already engineered by genetically linking viral antigens to its C-terminus. Each of the three test lines on LFA strip contains the proteinticle probes to detect disease-specific anti-viral antibodies. Compared to peptide probes, the proteinticle probes were evidently more sensitive, and the proteinticle probe-based LFA successfully diagnosed all the 20 patient sera per each disease without a false negative signal, whereas the diagnostic sensitivities in the peptide probe-based LFAs were 65-90%. Duplex and triplex assays performed with randomly mixed patient sera gave only true positive signals for all the 20 serum mixtures without any false positive signals, indicating 100% sensitivity and 100% specificity. It seems that on the proteinticle surface the antigenic peptides have homogeneous orientation and conformation without inter-peptide clustering and hence lead to the enhanced diagnostic performance with solving the problems of traditional diagnostic probes. Although the multiplex diagnosis of three viral diseases above was demonstrated as proof-of-concept here, the proposed LFA system can be applied to multiplex point of care diagnosis of other intractable diseases. (C) 2015 Elsevier B.V. All rights reserved.
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공과대학 (화공생명공학과)
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