The expression of 11 beta-hydroxysteroid dehydrogenase type 1 and 2 in nasal polyp-derived epithelial cells and its possible contribution to glucocorticoid activation in nasal polyp
DC Field | Value | Language |
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dc.contributor.author | Kook, Jin Ho | - |
dc.contributor.author | Kim, Hyun Jin | - |
dc.contributor.author | Kim, Kyung Won | - |
dc.contributor.author | Park, Se Jin | - |
dc.contributor.author | Kim, Tae Hoon | - |
dc.contributor.author | Lim, Sae Hee | - |
dc.contributor.author | Kang, Sung Hoon | - |
dc.contributor.author | Lee, Sang Hag | - |
dc.date.accessioned | 2021-09-04T14:48:24Z | - |
dc.date.available | 2021-09-04T14:48:24Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 1945-8924 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93147 | - |
dc.description.abstract | Background: The actions of glucocorticoids in target tissues depend on the local metabolism of glucocorticoids catalyzed by 11 beta hydroxysteroid dehydrogenase (HSD) 1 and 2. Glucocorticoids are the most effective anti-inflammatory drugs in the treatment of nasal polyps. However, the mechanisms that underlie the anti-inflammatory effects are unclear. Objective: The present study analyzed the expression of 11 beta-HSD1, 11 beta-HSD2, and steroidogenic enzymes (cytochrome P450, family 11, subfamily B, polypeptide 1 [CYP11B1]; cytochrome P450, family 11, subfamily A, polypeptide 1 [CYP11A1]) in nasal polyp tissues, and endogenous cortisol levels in nasal polyp-derived epithelial cells. Methods: The expression levels and distribution pattern of 11 beta-HSD1, 11 beta-HSD2, CYP11B1, and CYP11A1 were determined in nasal polyp tissues or nasal polyp-derived epithelial cells by using real-time polymerase chain reaction, Western blot, and immunohistochemistry testing. The expression levels of cortisol by using enzyme-linked immunosorbent assay were determined in cultured polyp-derived epithelial cells treated with adrenocorticotrophic hormone (ACTH), 11 beta-HSD1 inhibitor, or small interfering ribonucleic acid technique. The effect of glucocorticoids on the expression levels of these enzymes was investigated in cultured cells. Results: Expressed in nasal polyp tissues and nasal polyp-derived epithelial cells were 11 beta-HSD1, 11 beta-HSD2, CYP11B1, and CYP11A1. Cortisol production in cultured epithelial cells was decreased in cells treated with 11 beta-HSD1 small interfering ribonucleic acid or inhibitor, compared with nontreated cells. Cultured cells treated with adrenocorticotropic hormone induced increased cortisol production. 11 beta-HSD1 expression levels were upregulated in cells treated with glucocorticoid. Conclusions: Analysis of these results indicated that 11 beta-HSD1 expressed in polyp-derived epithelial cells may be involved in the anti-inflammatory function of glucocorticoid in the treatment of nasal polyps, which contributes to increased levels of endogenous cortisol. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OCEAN SIDE PUBLICATIONS INC | - |
dc.subject | UP-REGULATION | - |
dc.subject | CHRONIC RHINOSINUSITIS | - |
dc.subject | LOCALIZATION | - |
dc.subject | FIBROBLASTS | - |
dc.subject | MANAGEMENT | - |
dc.subject | CYTOKINES | - |
dc.subject | CORTISOL | - |
dc.subject | DISEASES | - |
dc.subject | ACTH | - |
dc.title | The expression of 11 beta-hydroxysteroid dehydrogenase type 1 and 2 in nasal polyp-derived epithelial cells and its possible contribution to glucocorticoid activation in nasal polyp | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Sang Hag | - |
dc.identifier.doi | 10.2500/ajra.2015.29.4185 | - |
dc.identifier.scopusid | 2-s2.0-84944036774 | - |
dc.identifier.wosid | 000359022100010 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, v.29, no.4, pp.246 - 250 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY | - |
dc.citation.title | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY | - |
dc.citation.volume | 29 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 246 | - |
dc.citation.endPage | 250 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | CHRONIC RHINOSINUSITIS | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | FIBROBLASTS | - |
dc.subject.keywordPlus | MANAGEMENT | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | CORTISOL | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordPlus | ACTH | - |
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