Neuroprotection against 6-OHDA-induced oxidative stress and apoptosis in SH-SY5Y cells by 5,7-Dihydroxychromone: Activation of the Nrf2/ARE pathway
- Authors
- Kim, Dong-Woo; Lee, Kyoung-tae; Kwon, Jaeyoung; Lee, Hak Ju; Lee, Dongho; Mar, Woongchon
- Issue Date
- 1-6월-2015
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- 5,7-Dihydroxychromone; Oxidative stress; 6-OHDA; Nrf2/ARE pathway; Neuroprotection; Cudrania tricuspidata
- Citation
- LIFE SCIENCES, v.130, pp.25 - 30
- Indexed
- SCIE
SCOPUS
- Journal Title
- LIFE SCIENCES
- Volume
- 130
- Start Page
- 25
- End Page
- 30
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/93289
- DOI
- 10.1016/j.lfs.2015.02.026
- ISSN
- 0024-3205
- Abstract
- Aims: The aim of this study was to prove the neuroprotective effect of 5,7-Dihydroxychromone (DHC) through the Nrf2/ARE signaling pathway. To elucidate the mechanism, we investigated whether 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in SH-SY5Y cells could be attenuated by DHC via activating the Nr12/ARE signal and whether DHC could down-regulate 6-OHDA-induced excessive ROS generation Main methods: To evaluate the neuroprotective effect of DHC against 6-OHDA-induced apoptosis, FACS analysis was performed using PI staining. The inhibitory effect of DHC against 6-OHDA-induced ROS generation was evaluated by DCFH-DA staining assay. Additionally, translocation of Nrf2 to the nucleus and increased Nrf2/ARE binding activity, which subsequently resulted in the up-regulation of the Nrf2-dependent antioxidant gene expressions including HO-1, NQO1, and GCLc, were evaluated by Western blotting and EMSA. Key findings: Pre-treatment of DHC, one of the constituents of Cudrania tricuspidata, significantly protects 6-OHDA-induced neuronal cell death and ROS generation. Also, DHC inhibited the expression of activated caspase-3 and caspase-9 and cleaved PARP in 6-OHDA-induced SH-SY5Y cells. DHC induced the translocation of Nrf2 to the nucleus and increased Nrf2/ARE binding activity which results in the up-regulation of the expression of Nrf2-dependent antioxidant genes, including HO-1, NQO1, and GCLc. The addition of Nrf2 siRNA abolished the neuroprotective effect of DHC against 6-OHDA-induced neurotoxicity and the expression of Nrf2-mediated antioxidant genes. Significance: Activation of Nrf2/ARE signal by DHC exerted neuroprotective effects against 6-OHDA-induced oxidative stress and apoptosis. This finding will give an insight that activating Nrf2/ARE signal could be a new potential therapeutic strategy for neurodegenerative disease. (C) 2015 Elsevier Inc. All rights reserved.
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