Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Different shapes of Al2O3 particles induce differential cytotoxicity via a mechanism involving lysosomal destabilization and reactive oxygen species generation

Authors
Kim, Byung IlJoo, Yong HoonPak, Pyo JuneKim, Joong-SuChung, Namhyun
Issue Date
6월-2015
Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
Keywords
Cytotoxicity; Interleukin-1beta; Lysosomal destabilization; Cathepsin B; Reactive oxygen species
Citation
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.58, no.3, pp.433 - 442
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY
Volume
58
Number
3
Start Page
433
End Page
442
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/93354
DOI
10.1007/s13765-015-0038-6
ISSN
1738-2203
Abstract
The biological effects of nano- and micro-sized Al2O3 particles are hypothesized to differ according to the shapes as well as the sizes of the particles. Thus, the mechanisms of interleukin (IL)-1 beta production and the association between the shape of the Al2O3 particle and its cytotoxicity in macrophage-like THP-1 cells were investigated using particles with three different shapes [N-Al2O3 (<30 nm), S-Al2O3 (2-4 nm x 100-800 nm), L-Al2O3 (2-4 nm 9 2800 nm)]. Levels of IL-1 beta production and cytotoxicity were concentration-dependent and were in the following order: S-Al2O3 > N-Al2O3 > L-Al2O3. Stimulus of THP-1 cells by Al2O3 particles led to lysosomal destabilization, generation of intracellular reactive oxygen species (ROS), and subsequent release of cathepsin B. The magnitude of the stimulus was dependent on the shapes and aspect ratios of the particles. Additional results suggested that caspase-1 (NALP3 inflammasome) activation and IL-1 beta production followed cathepsin B release. In addition, the cell death induced by Al2O3 particles was closely related with cathepsin B leakage. The mode of cell death was necrotic as much as apoptotic. We conclude that Al2O3 particles induce different levels of IL-1 beta production and cytotoxicity depending on their particle shapes or aspect ratios. The current finding may support the development of safe forms of Al2O3 particles.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biosystems and Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Chung, Nam hyun photo

Chung, Nam hyun
융합생명공학과
Read more

Altmetrics

Total Views & Downloads

BROWSE