PRMT4 is involved in insulin secretion via the methylation of histone H3 in pancreatic beta cells
- Authors
- Kim, Joong Kwan; Lim, Yongchul; Lee, Jung Ok; Lee, Young-Sun; Won, Nam Hee; Kim, Hyun; Kim, Hyeon Soo
- Issue Date
- 6월-2015
- Publisher
- BIOSCIENTIFICA LTD
- Keywords
- PRMT4; insulin synthesis; insulin secretion; histone H3R17
- Citation
- JOURNAL OF MOLECULAR ENDOCRINOLOGY, v.54, no.3, pp.315 - 324
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF MOLECULAR ENDOCRINOLOGY
- Volume
- 54
- Number
- 3
- Start Page
- 315
- End Page
- 324
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/93359
- DOI
- 10.1530/JME-14-0325
- ISSN
- 0952-5041
- Abstract
- The relationship between protein arginine methyltransferases (PRMTs) and insulin synthesis in beta cells is not yet well understood. In the present study, we showed that PRMT4 expression was increased in INS-1 and HIT-T15 pancreatic beta cells under high-glucose conditions. In addition, asymmetric dimethylation of Arg17 in histone H3 was significantly increased in both cell lines in the presence of glucose. The inhibition or knockdown of PRMT4 suppressed glucose-induced insulin gene expression in INS-1 cells by 81.6 and 79% respectively. Additionally, the overexpression of mutant PRMT4 also significantly repressed insulin gene expression. Consistently, insulin secretion induced in response to high levels of glucose was decreased by both PRMT4 inhibition and knockdown. Moreover, the inhibition of PRMT4 blocked high-glucose-induced insulin gene expression and insulin secretion in primary pancreatic islets. These results indicate that PRMT4 might be a key regulator of high-glucose-induced insulin secretion from pancreatic beta cells via H3R17 methylation.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.