Pepper aldehyde dehydrogenase CaALDH1 interacts with Xanthomonas effector AvrBsT and promotes effector-triggered cell death and defence responses
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Nak Hyun | - |
dc.contributor.author | Hwang, Byung Kook | - |
dc.date.accessioned | 2021-09-04T15:26:46Z | - |
dc.date.available | 2021-09-04T15:26:46Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-06 | - |
dc.identifier.issn | 0022-0957 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93361 | - |
dc.description.abstract | Xanthomonas type III effector AvrBsT induces hypersensitive cell death and defence responses in pepper (Capsicum annuum) and Nicotiana benthamiana. Little is known about the host factors that interact with AvrBsT. Here, we identified pepper aldehyde dehydrogenase 1 (CaALDH1) as an AvrBsT-interacting protein. Bimolecular fluorescence complementation and co-immunoprecipitation assays confirmed the interaction between CaALDH1 and AvrBsT in planta. CaALDH1: smGFP fluorescence was detected in the cytoplasm. CaALDH1 expression in pepper was rapidly and strongly induced by avirulent Xanthomonas campestris pv. vesicatoria (Xcv) Ds1 (avrBsT) infection. Transient co-expression of CaALDH1 with avrBsT significantly enhanced avrBsT-triggered cell death in N. benthamiana leaves. Aldehyde dehydrogenase activity was higher in leaves transiently expressing CaALDH1, suggesting that CaALDH1 acts as a cell death enhancer, independently of AvrBsT. CaALDH1 silencing disrupted phenolic compound accumulation, H2O2 production, defence response gene expression, and cell death during avirulent Xcv Ds1 (avrBsT) infection. Transgenic Arabidopsis thaliana overexpressing CaALDH1 exhibited enhanced defence response to Pseudomonas syringae pv. tomato and Hyaloperonospora arabidopsidis infection. These results indicate that cytoplasmic CaALDH1 interacts with AvrBsT and promotes plant cell death and defence responses. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | PLANT IMMUNE-SYSTEM | - |
dc.subject | HYPERSENSITIVE RESPONSE | - |
dc.subject | ARABIDOPSIS-THALIANA | - |
dc.subject | ABIOTIC STRESS | - |
dc.subject | GENOMIC STRUCTURE | - |
dc.subject | GENE SUPERFAMILY | - |
dc.subject | IN-PLANTA | - |
dc.subject | PROTEIN | - |
dc.subject | EXPRESSION | - |
dc.subject | PATHOGEN | - |
dc.title | Pepper aldehyde dehydrogenase CaALDH1 interacts with Xanthomonas effector AvrBsT and promotes effector-triggered cell death and defence responses | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Byung Kook | - |
dc.identifier.doi | 10.1093/jxb/erv147 | - |
dc.identifier.scopusid | 2-s2.0-84940664377 | - |
dc.identifier.wosid | 000356225400029 | - |
dc.identifier.bibliographicCitation | JOURNAL OF EXPERIMENTAL BOTANY, v.66, no.11, pp.3367 - 3380 | - |
dc.relation.isPartOf | JOURNAL OF EXPERIMENTAL BOTANY | - |
dc.citation.title | JOURNAL OF EXPERIMENTAL BOTANY | - |
dc.citation.volume | 66 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 3367 | - |
dc.citation.endPage | 3380 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | PLANT IMMUNE-SYSTEM | - |
dc.subject.keywordPlus | HYPERSENSITIVE RESPONSE | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | ABIOTIC STRESS | - |
dc.subject.keywordPlus | GENOMIC STRUCTURE | - |
dc.subject.keywordPlus | GENE SUPERFAMILY | - |
dc.subject.keywordPlus | IN-PLANTA | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PATHOGEN | - |
dc.subject.keywordAuthor | Aldehyde dehydrogenase | - |
dc.subject.keywordAuthor | cell death | - |
dc.subject.keywordAuthor | effector AvrBsT | - |
dc.subject.keywordAuthor | pepper | - |
dc.subject.keywordAuthor | plant defence | - |
dc.subject.keywordAuthor | Xanthomonas campestris pv. vesicatoria | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.