Enhanced autotrophic astaxanthin production from Haematococcus pluvialis under high temperature via heat stress-driven Haber-Weiss reaction
- Authors
- Hong, Min-Eui; Hwang, Sung Kwan; Chang, Won Seok; Kim, Byung Woo; Lee, Jeewon; Sim, Sang Jun
- Issue Date
- 6월-2015
- Publisher
- SPRINGER
- Keywords
- Haematococcus pluvialis; Photoautotrophic induction; Astaxanthin; High temperature; Haber-Weiss reaction
- Citation
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.99, no.12, pp.5203 - 5215
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 99
- Number
- 12
- Start Page
- 5203
- End Page
- 5215
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/93503
- DOI
- 10.1007/s00253-015-6440-5
- ISSN
- 0175-7598
- Abstract
- High temperatures (30-36 A degrees C) inhibited astaxanthin accumulation in Haematococcus pluvialis under photoautotrophic conditions. The depression of carotenogenesis was primarily attributed to excess intracellular less reactive oxygen species (LROS; O-2 (-) and H2O2) levels generated under high temperature conditions. Here, we show that the heat stress-driven inefficient astaxanthin production was improved by accelerating the iron-catalyzed Haber-Weiss reaction to convert LROS into more reactive oxygen species (MROS; O-2 and OH center dot), thereby facilitating lipid peroxidation. As a result, during 18 days of photoautotrophic induction, the astaxanthin concentration of cells cultured in high temperatures in the presence of iron (450 mu M) was dramatically increased by 75 % (30 A degrees C) and 133 % (36 A degrees C) compared to that of cells exposed to heat stress alone. The heat stress-driven Haber-Weiss reaction will be useful for economically producing astaxanthin by reducing energy cost and enhancing photoautotrophic astaxanthin production, particularly outdoors utilizing natural solar radiation including heat and light for photo-induction of H. pluvialis.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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