Epigenetic Alterations of IL-6/STAT3 Signaling by Placental Stem Cells Promote Hepatic Regeneration in a Rat Model with CCl4-induced Liver Injury
DC Field | Value | Language |
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dc.contributor.author | Jung, Jieun | - |
dc.contributor.author | Moon, Ji Wook | - |
dc.contributor.author | Choi, Jong-Ho | - |
dc.contributor.author | Lee, Yong Woo | - |
dc.contributor.author | Park, Sun-Hwa | - |
dc.contributor.author | Kim, Gi Jin | - |
dc.date.accessioned | 2021-09-04T16:48:34Z | - |
dc.date.available | 2021-09-04T16:48:34Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-05 | - |
dc.identifier.issn | 2005-3606 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93743 | - |
dc.description.abstract | Background: Human chorionic plate-derived mesenchymal stem cells (CP-MSCs) isolated from the placenta have been reported to demonstrate therapeutic effects in animal models of liver injury; however, the underlying epigenetic mechanism of this effect has not been elucidated. Thus, we investigated whether CP-MSCs influence epigenetic processes during regeneration of the injured liver. Methods: CP-MSCs were engrafted into a carbon tetrachloride (CCl4)-injured rat model through direct transplantation into the liver (DTX), intrasplenic transplantation (STX), and intravenous transplantation via the tail vein (TTX). Non-transplanted (NTX) rats were maintained as sham controls. Liver tissues were analyzed after transplantation using immunohistochemistry, western blot analysis, and quantitative methylation-specific polymerase chain reaction. Proliferation and human interleukin-6 (hIL-6) enzyme-linked immunosorbent assays were performed using CCl4-treated hepatic cells that were co-cultured with CP-MSCs. Results: The Ki67 labeling index, cell cyclins, albumin, IL-6, and gp130 levels were elevated in the CP-MSC transplantation groups. The concentration of hIL-6 in supernatants and the proliferation of CCl4-treated rat hepatic cells were enhanced by co-culturing with CF-MSCs (p < 0.05), while the methylation of IL-6/IL-6R and STAT3 by CP-MSC transplantation decreased. Conclusion: These results suggest that administration of CP-MSCs promotes IL-6/STAT3 signaling by decreasing the methylation of the IL-6/SATA3 promoters and thus inducing the proliferation of hepatic cells in a CCl4-injured liver rat model. These data advance our understanding of the therapeutic mechanisms. in injured livers, and can facilitate the development of cell-based therapies using placenta-derived stem cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC STEM CELL RESEARCH | - |
dc.subject | BONE-MARROW | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | DISEASES | - |
dc.subject | INTERLEUKIN-6 | - |
dc.subject | MICE | - |
dc.subject | PROLIFERATION | - |
dc.subject | HEPATECTOMY | - |
dc.subject | EXPRESSION | - |
dc.subject | MECHANISM | - |
dc.subject | RECIPIENT | - |
dc.title | Epigenetic Alterations of IL-6/STAT3 Signaling by Placental Stem Cells Promote Hepatic Regeneration in a Rat Model with CCl4-induced Liver Injury | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Sun-Hwa | - |
dc.identifier.doi | 10.15283/ijsc.2015.8.1.79 | - |
dc.identifier.scopusid | 2-s2.0-84930396802 | - |
dc.identifier.wosid | 000355638800009 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF STEM CELLS, v.8, no.1, pp.79 - 89 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF STEM CELLS | - |
dc.citation.title | INTERNATIONAL JOURNAL OF STEM CELLS | - |
dc.citation.volume | 8 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 79 | - |
dc.citation.endPage | 89 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordPlus | INTERLEUKIN-6 | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | HEPATECTOMY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | RECIPIENT | - |
dc.subject.keywordAuthor | DNA methylation | - |
dc.subject.keywordAuthor | IL-6 signaling | - |
dc.subject.keywordAuthor | Liver regeneration | - |
dc.subject.keywordAuthor | Placenta stem cells | - |
dc.subject.keywordAuthor | Transplantation routes | - |
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