Naturally-acquired cellular immune response against Plasmodium vivax merozoite surface protein-1 paralog antigen
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Changrob, Siriruk | - |
dc.contributor.author | Leepiyasakulchai, Chaniya | - |
dc.contributor.author | Tsuboi, Takafumi | - |
dc.contributor.author | Cheng, Yang | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.contributor.author | Chootong, Patchanee | - |
dc.contributor.author | Han, Eun-Taek | - |
dc.date.accessioned | 2021-09-04T17:15:52Z | - |
dc.date.available | 2021-09-04T17:15:52Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-04-15 | - |
dc.identifier.issn | 1475-2875 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93842 | - |
dc.description.abstract | Background: Plasmodium vivax merozoite surface protein-1 paralog (PvMSP1P) is a glycosylphosphatidylinositol-anchored protein expressed on the merozoite surface. This molecule is a target of natural immunity, as high anti-MSP1P-19 antibody levels were detected during P. vivax infection and the antibody inhibited PvMSP1P-erythrocyte binding. Recombinant PvMSP1P antigen results in production of a significant Th1 cytokine response in immunized mice. The present study was performed to characterize natural cellular immunity against PvMSP1P-19 and PvDBP region II in acute and recovery P. vivax infection. Methods: Peripheral blood mononuclear cells (PBMCs) from acute and recovery P. vivax infection were obtained for lymphocyte proliferation assay upon PvMSP1P-19 and PvDBP region II antigen stimulation. The culture supernatant was examined for the presence of the cytokines IL-2, TNF, IFN-gamma and IL-10 by enzyme-linked immunosorbent assay (ELISA). To determine whether Th1 or Th2 have a memory response against PvMSP1P-19 and PvDBPII protein antigen, PBMCs from subjects who had recovered from P. vivax infection 8-10 weeks prior to the study were obtained for lymphocyte proliferation assay. Cytokine-producing cells were analysed by flow cytometry. Results: IL-2 was detected at high levels in lymphocyte cultures from acutely infected P. vivax patients upon PvMSP1P-19 stimulation. Analysis of the Th1 or Th2 memory response in PBMC cultures from subjects who had recovered from P. vivax infection showed significantly elevated levels of PvMSP1P-19 and PvDBPII-specific IFN-gamma-producing cells (P < 0.05). Interestingly, the response of IFN-gamma-producing cells in PvMSP1P stimulation was fourfold greater in recovered subjects than that in acute-infection patients. CD4(+) T cells were the major cell phenotype involved in the response to PvMSP1P-19 and PvDBPII antigen. Conclusions: PvMSP1P-19 strongly induces a specific cellular immune response for protection against P. vivax compared with PvDBPII as the antigen induces activation of IFN-gamma-producing effector cells following natural P. vivax exposure. Upon stimulation, PvMSP1P-19 has the potential to activate the recall response of Th1 effector memory cells that play a role in killing the parasite. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | BLOOD-STAGE MALARIA | - |
dc.subject | LIGAND DOMAIN | - |
dc.subject | ENDEMIC AREA | - |
dc.subject | T-CELLS | - |
dc.subject | FALCIPARUM | - |
dc.subject | IMMUNOGENICITY | - |
dc.subject | INDIVIDUALS | - |
dc.subject | PROTECTION | - |
dc.subject | INDUCTION | - |
dc.subject | DIVERSITY | - |
dc.title | Naturally-acquired cellular immune response against Plasmodium vivax merozoite surface protein-1 paralog antigen | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Chae Seung | - |
dc.identifier.doi | 10.1186/s12936-015-0681-8 | - |
dc.identifier.wosid | 000353201100001 | - |
dc.identifier.bibliographicCitation | MALARIA JOURNAL, v.14 | - |
dc.relation.isPartOf | MALARIA JOURNAL | - |
dc.citation.title | MALARIA JOURNAL | - |
dc.citation.volume | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Infectious Diseases | - |
dc.relation.journalResearchArea | Parasitology | - |
dc.relation.journalResearchArea | Tropical Medicine | - |
dc.relation.journalWebOfScienceCategory | Infectious Diseases | - |
dc.relation.journalWebOfScienceCategory | Parasitology | - |
dc.relation.journalWebOfScienceCategory | Tropical Medicine | - |
dc.subject.keywordPlus | BLOOD-STAGE MALARIA | - |
dc.subject.keywordPlus | LIGAND DOMAIN | - |
dc.subject.keywordPlus | ENDEMIC AREA | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | FALCIPARUM | - |
dc.subject.keywordPlus | IMMUNOGENICITY | - |
dc.subject.keywordPlus | INDIVIDUALS | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordAuthor | Plasmodium vivax | - |
dc.subject.keywordAuthor | PvMSP1P | - |
dc.subject.keywordAuthor | Cellular immune response | - |
dc.subject.keywordAuthor | Patients | - |
dc.subject.keywordAuthor | Infection | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.