Increased expression of p21-activated kinase 4 in adenomyosis and its regulation of matrix metalloproteinase-2 and-9 in endometrial cells
DC Field | Value | Language |
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dc.contributor.author | Yi, Kyong Wook | - |
dc.contributor.author | Kim, Sung Hoon | - |
dc.contributor.author | Ihm, Hyo Jin | - |
dc.contributor.author | Oh, Young Sang | - |
dc.contributor.author | Chae, Hee Dong | - |
dc.contributor.author | Kim, Chung-Hoon | - |
dc.contributor.author | Kang, Byung Moon | - |
dc.date.accessioned | 2021-09-04T17:34:35Z | - |
dc.date.available | 2021-09-04T17:34:35Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 0015-0282 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93914 | - |
dc.description.abstract | Objective: To investigate the expression of p21-activated kinase 4 (Pak4) in both adenomyotic foci and the eutopic endometrium of women with adenomyosis, and whether the activities of matrix metalloproteinases (MMPs)-2 and -9 are regulated by Pak4 in endometrial cells. Design: Experimental study using human samples and cell lines. Setting: University hospital. Patient(s): Thirty-nine patients with histologic evidence of adenomyosis, and 34 patients with carcinoma in situ of the uterine cervix without adenomyosis or endometriosis. Intervention(s): Immunohistochemistry, zymography after transfection with Pak4 small interfering RNA (siRNA), and western blot analyses after nuclear factor kappa-B (NF-kappa B) inhibitor treatment. Main Outcome Measure(s): The Pak4 immunoreactivity of women with vs. without adenomyosis was compared semiquantitatively. The activities of MMP-2 and -9 were analyzed in eutopic endometrial stromal cells and Ishikawa cells after transfection with Pak4 siRNA. The Pak4 expression was evaluated in endometrial cells after treatment with NF-kappa B inhibitor. Result(s): Pak4 immunoreactivity was increased in adenomyotic foci and in the eutopic endometrium of women with adenomyosis. Transfection of endometrial cells with Pak4 siRNA led to significant decreases of MMP-2 and -9 activities. In vitro treatment of endometrial cells with tumor necrosis factor-alpha caused a significant increase of NF-kappa B activation and Pak4 expression, which was obviously decreased by the NF-kappa B inhibitor pyrrolidinedithiocarbamate. Conclusion(s): Our results suggest that Pak4 is regulated by NF-kappa B and that increased Pak4 expression can lead to development of adenomyosis by enhancing the invasiveness of endometrial cells through regulation of MMP-2 and -9 activities. (C) 2015 by American Society for Reproductive Medicine. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | FACTOR-KAPPA-B | - |
dc.subject | NECROSIS-FACTOR-ALPHA | - |
dc.subject | EUTOPIC ENDOMETRIUM | - |
dc.subject | TISSUE INHIBITOR | - |
dc.subject | WOMEN | - |
dc.subject | CANCER | - |
dc.subject | MIGRATION | - |
dc.subject | INVASION | - |
dc.subject | PAK4 | - |
dc.subject | CYCLOOXYGENASE-2 | - |
dc.title | Increased expression of p21-activated kinase 4 in adenomyosis and its regulation of matrix metalloproteinase-2 and-9 in endometrial cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yi, Kyong Wook | - |
dc.identifier.doi | 10.1016/j.fertnstert.2014.12.124 | - |
dc.identifier.scopusid | 2-s2.0-84930809510 | - |
dc.identifier.wosid | 000352110400040 | - |
dc.identifier.bibliographicCitation | FERTILITY AND STERILITY, v.103, no.4, pp.1089 - U303 | - |
dc.relation.isPartOf | FERTILITY AND STERILITY | - |
dc.citation.title | FERTILITY AND STERILITY | - |
dc.citation.volume | 103 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1089 | - |
dc.citation.endPage | U303 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Obstetrics & Gynecology | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Obstetrics & Gynecology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.subject.keywordPlus | FACTOR-KAPPA-B | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | EUTOPIC ENDOMETRIUM | - |
dc.subject.keywordPlus | TISSUE INHIBITOR | - |
dc.subject.keywordPlus | WOMEN | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordPlus | PAK4 | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordAuthor | P21-activated kinase 4 (Pak4) | - |
dc.subject.keywordAuthor | adenomyosis | - |
dc.subject.keywordAuthor | endometrium | - |
dc.subject.keywordAuthor | matrix metalloproteinase (MMP) | - |
dc.subject.keywordAuthor | nuclear factor kappa-B (NF-kappa B) | - |
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