Structure of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus in Complex with a Protective Monoclonal Antibody
DC Field | Value | Language |
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dc.contributor.author | Cho, Ki Joon | - |
dc.contributor.author | Schepens, Bert | - |
dc.contributor.author | Seok, Jong Hyeon | - |
dc.contributor.author | Kim, Sella | - |
dc.contributor.author | Roose, Kenny | - |
dc.contributor.author | Lee, Ji-Hye | - |
dc.contributor.author | Gallardo, Rodrigo | - |
dc.contributor.author | Van Hamme, Evelien | - |
dc.contributor.author | Schymkowitz, Joost | - |
dc.contributor.author | Rousseau, Frederic | - |
dc.contributor.author | Fiers, Walter | - |
dc.contributor.author | Saelens, Xavier | - |
dc.contributor.author | Kim, Kyung Hyun | - |
dc.date.accessioned | 2021-09-04T17:39:54Z | - |
dc.date.available | 2021-09-04T17:39:54Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93951 | - |
dc.description.abstract | The extracellular domain of influenza A virus matrix protein 2 (M2e) is conserved and is being evaluated as a quasiuniversal influenza A vaccine candidate. We describe the crystal structure at 1.6 angstrom resolution of M2e in complex with the Fab fragment of an M2e-specific monoclonal antibody that protects against influenza A virus challenge. This antibody binds M2 expressed on the surfaces of cells infected with influenza A virus. Five out of six complementary determining regions interact with M2e, and three highly conserved M2e residues are critical for this interaction. In this complex, M2e adopts a compact U-shaped conformation stabilized in the center by the highly conserved tryptophan residue in M2e. This is the first description of the three-dimensional structure of M2e. IMPORTANCE M2e of influenza A is under investigation as a universal influenza A vaccine, but its three-dimensional structure is unknown. We describe the structure of M2e stabilized with an M2e-specific monoclonal antibody that recognizes natural M2. We found that the conserved tryptophan is positioned in the center of the U-shaped structure of M2e and stabilizes its conformation. The structure also explains why previously reported in vivo escape viruses, selected with a similar monoclonal antibody, carried proline residue substitutions at position 10 in M2. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | M2 PROTEIN | - |
dc.subject | SOLUTION CONFORMATION | - |
dc.subject | IMMUNOGENIC PEPTIDE | - |
dc.subject | PROTON CHANNEL | - |
dc.subject | ECTODOMAIN | - |
dc.subject | MECHANISM | - |
dc.subject | HEMAGGLUTININ | - |
dc.subject | REPLICATION | - |
dc.subject | SPECIFICITY | - |
dc.subject | CRYSTAL | - |
dc.title | Structure of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus in Complex with a Protective Monoclonal Antibody | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hyun | - |
dc.identifier.doi | 10.1128/JVI.02576-14 | - |
dc.identifier.scopusid | 2-s2.0-84924769564 | - |
dc.identifier.wosid | 000352216100021 | - |
dc.identifier.bibliographicCitation | JOURNAL OF VIROLOGY, v.89, no.7, pp.3700 - 3711 | - |
dc.relation.isPartOf | JOURNAL OF VIROLOGY | - |
dc.citation.title | JOURNAL OF VIROLOGY | - |
dc.citation.volume | 89 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 3700 | - |
dc.citation.endPage | 3711 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | M2 PROTEIN | - |
dc.subject.keywordPlus | SOLUTION CONFORMATION | - |
dc.subject.keywordPlus | IMMUNOGENIC PEPTIDE | - |
dc.subject.keywordPlus | PROTON CHANNEL | - |
dc.subject.keywordPlus | ECTODOMAIN | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | HEMAGGLUTININ | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | CRYSTAL | - |
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