Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-kappa B signaling
DC Field | Value | Language |
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dc.contributor.author | Park, Ji-Won | - |
dc.contributor.author | Kwon, Ok-Kyoung | - |
dc.contributor.author | Kim, Jung-Hee | - |
dc.contributor.author | Oh, Sei-Ryang | - |
dc.contributor.author | Kim, Jae-Hong | - |
dc.contributor.author | Paik, Jin-Hyub | - |
dc.contributor.author | Marwoto, Bambang | - |
dc.contributor.author | Widjhati, Rifatul | - |
dc.contributor.author | Juniarti, Fifit | - |
dc.contributor.author | Irawan, Doddy | - |
dc.contributor.author | Ahn, Kyung-Seop | - |
dc.date.accessioned | 2021-09-04T17:58:29Z | - |
dc.date.available | 2021-09-04T17:58:29Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94052 | - |
dc.description.abstract | Rhododendron album Blume (RA) has traditionally been used as an herbal medicine and is considered to have anti-inflammatory properties. In the present study, we screened RA extracts with anti-inflammatory properties. The biological effects of an RA methanol extract (RAME) on inflammation were investigated in lipopolysaccharide (LPS)-stimulated mouse RAW264.7 cells. We investigated the effects of RAME on the production of nitric oxide (NO) and prostaglandin E2 (PGE(2)) in LPS-stimulated RAW264.7 cells. To explore the anti-inflammatory mechanisms of RAME, we measured the mRNA and protein expression of pro-inflammatory mediators induced by RAME in the LPS-stimulated RAW264.7 cells by RT-PCR and western blot analysis, respectively. RAME significantly inhibited the production of NO, PGE(2), interleukin (IL)-6, IL-1 beta and tumor necrosis factor (TNF)-alpha in the LPS-stimulated RAW264.7 cells. It also suppressed the mRNA and protein expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and mitogenactivated protein kinases (MAPKs) with a concomitant decrease in the nuclear translocation of nuclear factor-kappa B (NF-kappa B) in the LPS-stimulated RAW264.7 cells. These results indicate that RAME inhibits LPS-induced inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-kappa B activation. We therefore suggest that RAME may be prove to be an effective therapeutic agent for the treatment of inflammatory diseases. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | NITRIC-OXIDE SYNTHASE | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | MURINE MACROPHAGES | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | INNATE IMMUNITY | - |
dc.subject | HUMAN MONOCYTES | - |
dc.subject | INFLAMMATION | - |
dc.subject | CANCER | - |
dc.subject | P38 | - |
dc.title | Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-kappa B signaling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jae-Hong | - |
dc.identifier.doi | 10.3892/ijmm.2015.2107 | - |
dc.identifier.scopusid | 2-s2.0-84923677663 | - |
dc.identifier.wosid | 000351764800017 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.35, no.4, pp.987 - 994 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.volume | 35 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 987 | - |
dc.citation.endPage | 994 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | MURINE MACROPHAGES | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | INNATE IMMUNITY | - |
dc.subject.keywordPlus | HUMAN MONOCYTES | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | P38 | - |
dc.subject.keywordAuthor | Rhododendron album Blume | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | lipopolysaccharide | - |
dc.subject.keywordAuthor | mitogen-activated protein kinases | - |
dc.subject.keywordAuthor | nuclear factor-kappa B | - |
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