A Highly Efficient Recombinant Laccase from the Yeast Yarrowia lipolytica and Its Application in the Hydrolysis of Biomass
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kalyani, Dayanand | - |
dc.contributor.author | Tiwari, Manish Kumar | - |
dc.contributor.author | Li, Jinglin | - |
dc.contributor.author | Kim, Sun Chang | - |
dc.contributor.author | Kalia, Vipin C. | - |
dc.contributor.author | Kang, Yun Chan | - |
dc.contributor.author | Lee, Jung-Kul | - |
dc.date.accessioned | 2021-09-04T18:07:51Z | - |
dc.date.available | 2021-09-04T18:07:51Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-03-17 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94112 | - |
dc.description.abstract | A modified thermal asymmetric interlaced polymerase chain reaction was performed to obtain the first yeast laccase gene (YlLac) from the isolated yeast Yarrowia lipolytica. The 1557-bp full-length cDNA of YlLac encoded a mature laccase protein containing 519 amino acids preceded by a signal peptide of 19 amino acids, and the YlLac gene was expressed in the yeast Pichia pastoris. YlLac is a monomeric glycoprotein with a molecular mass of similar to 55 kDa as determined by polyacrylamide-gel electrophoresis. It showed a higher catalytic efficiency towards 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (k(cat)/K-m = 17.5 s(-1) mu M-1) and 2,6-dimethoxyphenol (k(cat)/K-m = 16.1 s(-1) mu M-1) than other reported laccases. The standard redox potential of the T1 site of the enzyme was found to be 772 mV. The highest catalytic efficiency of the yeast recombinant laccase, YlLac, makes it a good candidate for industrial applications: it removes phenolic compounds in acid-pretreated woody biomass (Populus balsamifera) and enhanced saccharification. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | ENZYMATIC-HYDROLYSIS | - |
dc.subject | MULTICOPPER OXIDASE | - |
dc.subject | PHENOLIC-COMPOUNDS | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | RICE STRAW | - |
dc.subject | PURIFICATION | - |
dc.subject | TECHNOLOGIES | - |
dc.subject | PRETREATMENT | - |
dc.subject | SUBSTRATE | - |
dc.title | A Highly Efficient Recombinant Laccase from the Yeast Yarrowia lipolytica and Its Application in the Hydrolysis of Biomass | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kang, Yun Chan | - |
dc.identifier.doi | 10.1371/journal.pone.0120156 | - |
dc.identifier.scopusid | 2-s2.0-84924940447 | - |
dc.identifier.wosid | 000351284600133 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.10, no.3 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 10 | - |
dc.citation.number | 3 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | ENZYMATIC-HYDROLYSIS | - |
dc.subject.keywordPlus | MULTICOPPER OXIDASE | - |
dc.subject.keywordPlus | PHENOLIC-COMPOUNDS | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | RICE STRAW | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | TECHNOLOGIES | - |
dc.subject.keywordPlus | PRETREATMENT | - |
dc.subject.keywordPlus | SUBSTRATE | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.