Label-free Electrochemical Detection of the Human Adenovirus 40/41 Fiber Gene
DC Field | Value | Language |
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dc.contributor.author | Song, Min-Jung | - |
dc.contributor.author | Jin, Joon-Hyung | - |
dc.date.accessioned | 2021-09-04T18:42:26Z | - |
dc.date.available | 2021-09-04T18:42:26Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 0910-6340 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94278 | - |
dc.description.abstract | A nanoporous silicon-based label-free DNA biosensor was fabricated to monitor rapidly enteric adenovirus types 40 and 41, a leading cause of viral gastroenteritis in children. Nanoporous silicon (NPS) was formed by an anodic etching process in a mixture solution containing hydrofluoric acid and ethanol. The polypyrrole (PPy) film was directly electropolymerized on The NPS substrate. Twenty-five base pairs of probe DNA (pDNA), derived from the fiber gene, was electrochemically doped on the PPy-coated NPS substrate. The conductivity change due to the immobilized pDNA and hybridized target DNA (tDNA) was expressed as an arbitrary factor, gamma, which is a normalized numerical term used for the selective quantification of the tDNA. gamma was inversely proportional to the concentration of complementary tDNA, but independent of the non-complementary tDNA. The sensitivity slope for detecting tDNA(c) was -1.54 mu M-1, based on the factor gamma in the range of 0.4 to 1.0 mu M of tDNA. The surface roughness was characterized using atomic force microscopy. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | JAPAN SOC ANALYTICAL CHEMISTRY | - |
dc.subject | ELECTRONIC DETECTION | - |
dc.subject | DNA-HYBRIDIZATION | - |
dc.subject | POLYPYRROLE | - |
dc.subject | BIOSENSOR | - |
dc.subject | QUANTIFICATION | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | PREVALENCE | - |
dc.subject | GLASS | - |
dc.title | Label-free Electrochemical Detection of the Human Adenovirus 40/41 Fiber Gene | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jin, Joon-Hyung | - |
dc.identifier.doi | 10.2116/analsci.31.159 | - |
dc.identifier.scopusid | 2-s2.0-84930240908 | - |
dc.identifier.wosid | 000351566300004 | - |
dc.identifier.bibliographicCitation | ANALYTICAL SCIENCES, v.31, no.3, pp.159 - 163 | - |
dc.relation.isPartOf | ANALYTICAL SCIENCES | - |
dc.citation.title | ANALYTICAL SCIENCES | - |
dc.citation.volume | 31 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 159 | - |
dc.citation.endPage | 163 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | ELECTRONIC DETECTION | - |
dc.subject.keywordPlus | DNA-HYBRIDIZATION | - |
dc.subject.keywordPlus | POLYPYRROLE | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | PREVALENCE | - |
dc.subject.keywordPlus | GLASS | - |
dc.subject.keywordAuthor | Direct electropolymerization | - |
dc.subject.keywordAuthor | coulometric DNA sensor | - |
dc.subject.keywordAuthor | human adenovirus | - |
dc.subject.keywordAuthor | nanoporous silicon | - |
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