A Microreactor System for Cultivation of Haematococcus pluvialis and Astaxanthin Production
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwak, Ho Seok | - |
dc.contributor.author | Kim, Jaoon Young Hwan | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.date.accessioned | 2021-09-04T19:44:33Z | - |
dc.date.available | 2021-09-04T19:44:33Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94564 | - |
dc.description.abstract | Development of efficient culture and monitoring system for cell growth and production of useful materials is required for practical utilization of microalgae. In the present study, we developed a PDMS-based microreactor system for efficient, rapid culture of microalgae and monitoring of cell growth, carotenoid content under diverse culture conditions. Due to advantages of PDMS, we optimized culture conditions (light intensity, pH, nitrate depletion, carbon dioxide concentration) for improving growth rate and astaxanthin productivity in considerably less time compared to conventional culture methods using flask or well plate. In addition, we found that there was a strong linear correlation between fluorescence intensity of astaxanthin stained by Nile red and the astaxanthin content, which can be utilized as a high-throughput screening tool in microfluidic systems. In this study, the growth rate of vegetative Haematococcus pluvialis was improved by 60% in microfluidic chamber than in flask and astaxanthin was produced up to 362 mg/L under the optimal conditions (300 mu mol photon/m(2)/s of light, 7% CO2 (v/v), and pH 7.0) using designed microfluidic devices. This result shows that microfluidic system can provide effective means to address development of microalgal strains including H. pluvialis and bioprocess. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.subject | GREEN-ALGA | - |
dc.subject | MICROFLUIDICS | - |
dc.subject | TECHNOLOGIES | - |
dc.subject | CELLS | - |
dc.subject | RATIO | - |
dc.title | A Microreactor System for Cultivation of Haematococcus pluvialis and Astaxanthin Production | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1166/jnn.2015.9321 | - |
dc.identifier.scopusid | 2-s2.0-84920773992 | - |
dc.identifier.wosid | 000345054100105 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.15, no.2, pp.1618 - 1623 | - |
dc.relation.isPartOf | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 15 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1618 | - |
dc.citation.endPage | 1623 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | GREEN-ALGA | - |
dc.subject.keywordPlus | MICROFLUIDICS | - |
dc.subject.keywordPlus | TECHNOLOGIES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | RATIO | - |
dc.subject.keywordAuthor | Astaxanthin | - |
dc.subject.keywordAuthor | Haematococcus pluvialis | - |
dc.subject.keywordAuthor | Microfluidic Devices | - |
dc.subject.keywordAuthor | Nile Red | - |
dc.subject.keywordAuthor | PDMS | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.