Optical and structural properties of plasma-treated Cordyceps bassiana spores as studied by circular dichroism, absorption, and fluorescence spectroscopy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Geon Joon | - |
dc.contributor.author | Sim, Geon Bo | - |
dc.contributor.author | Choi, Eun Ha | - |
dc.contributor.author | Kwon, Young-Wan | - |
dc.contributor.author | Kim, Jun Young | - |
dc.contributor.author | Jang, Siun | - |
dc.contributor.author | Kim, Seong Hwan | - |
dc.date.accessioned | 2021-09-04T20:03:46Z | - |
dc.date.available | 2021-09-04T20:03:46Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015-01-14 | - |
dc.identifier.issn | 0021-8979 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94646 | - |
dc.description.abstract | To understand the killing mechanism of fungal spores by plasma treatment, the optical, structural, and biological properties of the insect pathogenic fungus Cordyceps bassiana spores were studied. A nonthermal atmospheric-pressure plasma jet (APPJ) was used to treat the spores in aqueous solution. Optical emission spectra of the APPJ acquired in air indicated emission peaks corresponding to hydroxyl radicals and atomic oxygen. When the APPJ entered the aqueous solution, additional reactive species were derived from the interaction of plasma radicals with the aqueous solution. Fluorescence and absorption spectroscopy confirmed the generation of hydroxyl radicals and hydrogen peroxide in the plasma-activated water (PAW). Spore counting showed that plasma treatment significantly reduced spore viability. Absorption spectroscopy, circular dichroism (CD) spectroscopy, and agarose gel electrophoresis of the DNA extracted from plasma-treated spores showed a reduction in spore DNA content. The magnitude of the dip in the CD spectrum was lower in the plasma-treated spores than in the control, indicating that plasma treatment causes structural modifications and/or damage to cellular components. Tryptophan fluorescence intensity was lower in the plasma-treated spores than in the control, suggesting that plasma treatment modified cell wall proteins. Changes in spore viability and DNA content were attributed to structural modification of the cell wall by reactive species coming from the APPJ and the PAW. Our results provided evidence that the plasma radicals and the derived reactive species play critical roles in fungal spore inactivation. (C) 2015 AIP Publishing LLC. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER INST PHYSICS | - |
dc.subject | FUNGUS BEAUVERIA-BASSIANA | - |
dc.subject | ENTOMOPATHOGENIC FUNGUS | - |
dc.subject | INTRINSIC FLUORESCENCE | - |
dc.subject | RAMAN-SPECTRUM | - |
dc.subject | INACTIVATION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | HYDROGEN | - |
dc.subject | DNA | - |
dc.subject | STERILIZATION | - |
dc.subject | RESPONSES | - |
dc.title | Optical and structural properties of plasma-treated Cordyceps bassiana spores as studied by circular dichroism, absorption, and fluorescence spectroscopy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kwon, Young-Wan | - |
dc.identifier.doi | 10.1063/1.4905194 | - |
dc.identifier.scopusid | 2-s2.0-84923646251 | - |
dc.identifier.wosid | 000348129300021 | - |
dc.identifier.bibliographicCitation | JOURNAL OF APPLIED PHYSICS, v.117, no.2 | - |
dc.relation.isPartOf | JOURNAL OF APPLIED PHYSICS | - |
dc.citation.title | JOURNAL OF APPLIED PHYSICS | - |
dc.citation.volume | 117 | - |
dc.citation.number | 2 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | FUNGUS BEAUVERIA-BASSIANA | - |
dc.subject.keywordPlus | ENTOMOPATHOGENIC FUNGUS | - |
dc.subject.keywordPlus | INTRINSIC FLUORESCENCE | - |
dc.subject.keywordPlus | RAMAN-SPECTRUM | - |
dc.subject.keywordPlus | INACTIVATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | HYDROGEN | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | STERILIZATION | - |
dc.subject.keywordPlus | RESPONSES | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.