Longitudinal monitoring adipose-derived stem cell survival by PET imaging hexadecyl-4-I-124-iodobenzoate in rat myocardial infarction model
- Authors
- Kim, Min Hwan; Woo, Sang-Keun; Lee, Kyo Chul; An, Gwang Il; Pandya, Darpan; Park, Noh Won; Nahm, Sang-Soep; Eom, Ki Dong; Kim, Kwang Il; Lee, Tae Sup; Kim, Chan Wha; Kang, Joo Hyun; Yoo, Jeongsoo; Lee, Yong Jin
- Issue Date
- 2-1월-2015
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Adipose-derived stem cells; Cell tracking; Direct labeling agent; Hexadecyl-4-I-124-iodobenzoate; Myocardial infarction
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.456, no.1, pp.13 - 19
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 456
- Number
- 1
- Start Page
- 13
- End Page
- 19
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/94668
- DOI
- 10.1016/j.bbrc.2014.11.019
- ISSN
- 0006-291X
- Abstract
- This study aims to monitor how the change of cell survival of transplanted adipose-derived stem cells (ADSCs) responds to myocardial infarction (MI) via the hexadecyl-4-I-124-iodobenzoate (I-124-HIB) mediated direct labeling method in vivo. Stem cells have shown the potential to improve cardiac function after MI. However, monitoring of the fate of transplanted stem cells at target sites is still unclear. Rat ADSCs were labeled with I-124-HIB, and radiolabeled ADSCs were transplanted into the myocardium of normal and MI model. In the group of I-124-HIB-labeled ADSC transplantation, in vivo imaging was performed using small-animal positron emission tomography (PET)/computed tomography (CT) for 9 days. Twenty-one days post-transplantation, histopathological analysis and apoptosis assay were performed. ADSC viability and differentiation were not affected by I-124-HIB labeling. In vivo tracking of the I-124-HIB-labeled ADSCs was possible for 9 and 3 days in normal and MI model, respectively. Apoptosis of transplanted cells increased in the MI model compared than that in normal model. We developed a direct labeling agent, I-124-HIB, and first tried to longitudinally monitor transplanted stem cell to MI. This approach may provide new insights on the roles of stem cell monitoring in living bodies for stem cell therapy from pre-clinical studies to clinical trials. (C) 2014 Elsevier Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholar.korea.ac.kr/handle/2021.sw.korea/94668)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.