Epigallocatechin-3-gallate suppresses the lipid deposition through the apoptosis during differentiation in bovine bone marrow mesenchymal stem cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Jin Young | - |
dc.contributor.author | Suresh, Sekar | - |
dc.contributor.author | Jang, Mi | - |
dc.contributor.author | Park, Mi Na | - |
dc.contributor.author | Gobianand, Kuppannan | - |
dc.contributor.author | You, Seungkwon | - |
dc.contributor.author | Yeon, Sung-Heom | - |
dc.contributor.author | Lee, Hyun-Jeong | - |
dc.date.accessioned | 2021-09-04T20:24:13Z | - |
dc.date.available | 2021-09-04T20:24:13Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 1065-6995 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94745 | - |
dc.description.abstract | Epigallocatechin gallate (EGCG), a major component of tea, has known effects on obesity, fatty liver, and obesity-related cancer. We explored the effects of EGCG on the differentiation of bovine mesenchymal stem cells (BMSCs, which are multipotent) in a dose- and time-dependent manner. Differentiating BMSCs were exposed to various concentrations of EGCG (0, 10, 50, 100, and 200 mu M) for 2, 4, and 6 days. BMSCs were cultured in Dulbecco's modified Eagle's medium (DMEM)/high-glucose medium with adipogenic inducers for 6 days, and the expression levels of various genes involved in adipogenesis were measured using real-time polymerase chain reaction (PCR) and Western blotting. We assessed apoptosis by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining of control and EGCG-exposed cells. We found that EGCG significantly suppressed fat deposition and cell viability (P<0.05). The mRNA and protein levels of various adipogenic factors were measured. Expression of the genes encoding peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer-binding protein alpha (CEBPA), fatty acid-binding protein 4 (FABP4), and stearoyl-CoA desaturase (SCD) were diminished by EGCG during adipogenic differentiation (P<0.05). We also found that EGCG lowered the expression levels of the adipogenic proteins encoded by these genes (P<0.05). EGCG induced apoptosis during adipogenic differentiation (P<0.05). Thus, exposure to EGCG potentially inhibits adipogenesis by triggering apoptosis; the data suggest that EGCG inhibits adipogenic differentiation in BMSCs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | HUMAN ADIPOSE-TISSUE | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | STROMAL CELLS | - |
dc.subject | OSTEOGENIC DIFFERENTIATION | - |
dc.subject | 3T3-L1 CELLS | - |
dc.subject | MCF-7 CELLS | - |
dc.subject | IN-VIVO | - |
dc.subject | GALLATE | - |
dc.subject | EGCG | - |
dc.subject | (-)-EPIGALLOCATECHIN-3-GALLATE | - |
dc.title | Epigallocatechin-3-gallate suppresses the lipid deposition through the apoptosis during differentiation in bovine bone marrow mesenchymal stem cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | You, Seungkwon | - |
dc.identifier.doi | 10.1002/cbin.10343 | - |
dc.identifier.scopusid | 2-s2.0-84921062372 | - |
dc.identifier.wosid | 000347382000007 | - |
dc.identifier.bibliographicCitation | CELL BIOLOGY INTERNATIONAL, v.39, no.1, pp.52 - 64 | - |
dc.relation.isPartOf | CELL BIOLOGY INTERNATIONAL | - |
dc.citation.title | CELL BIOLOGY INTERNATIONAL | - |
dc.citation.volume | 39 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 52 | - |
dc.citation.endPage | 64 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | HUMAN ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | OSTEOGENIC DIFFERENTIATION | - |
dc.subject.keywordPlus | 3T3-L1 CELLS | - |
dc.subject.keywordPlus | MCF-7 CELLS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | GALLATE | - |
dc.subject.keywordPlus | EGCG | - |
dc.subject.keywordPlus | (-)-EPIGALLOCATECHIN-3-GALLATE | - |
dc.subject.keywordAuthor | adipogenic factor | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | BMSC | - |
dc.subject.keywordAuthor | differentiation | - |
dc.subject.keywordAuthor | EGCG | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.