Configurational changes of heme followed by cytochrome c folding reaction
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Jungkweon | - |
dc.contributor.author | Cho, Dae Won | - |
dc.contributor.author | Tojo, Sachiko | - |
dc.contributor.author | Fujitsuka, Mamoru | - |
dc.contributor.author | Majima, Tetsuro | - |
dc.date.accessioned | 2021-09-04T20:33:00Z | - |
dc.date.available | 2021-09-04T20:33:00Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 1742-206X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94807 | - |
dc.description.abstract | Although the folding kinetics of cytochrome c (Cyt-c), ferric or ferrous Cyt-c, has been extensively investigated as a paradigm for a protein folding reaction using various time-resolved spectroscopic techniques, the configurational change of heme associated with the folding reaction from a ferric Cyt-c to a ferrous Cyt-c induced by one-electron reduction has not been elucidated. To address this issue, we investigated the configurational change of heme in the Cyt-c folding process induced by one-electron reduction using a combination of time-resolved resonance Raman spectroscopy and pulse radiolysis. The results presented herein reveal that the reduction of ferric Cyt-c and the ligation of Met80 occur simultaneously within a timescale of approximately 2 mu s, and that the ligand binding and exchange of heme depend on the initial configuration of the heme. The rapid ligation of Met80 observed in this study may be attributed to the intramolecular diffusion of Met80 into ferrous Cyt-c with a 5-coordinated high-spin configuration. Conversely, the ligand exchange of a ferrous Cyt-c with a 6-coordinated low-spin configuration was significantly slower. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | RESOLVED CIRCULAR-DICHROISM | - |
dc.subject | ELECTRON-TRANSFER | - |
dc.subject | PULSE-RADIOLYSIS | - |
dc.subject | RESONANCE RAMAN | - |
dc.subject | SPEED LIMIT | - |
dc.subject | TRANSIENT ABSORPTION | - |
dc.subject | PROTEIN | - |
dc.subject | DYNAMICS | - |
dc.subject | TIME | - |
dc.subject | SPECTROSCOPY | - |
dc.title | Configurational changes of heme followed by cytochrome c folding reaction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Dae Won | - |
dc.identifier.doi | 10.1039/c4mb00551a | - |
dc.identifier.scopusid | 2-s2.0-84918814401 | - |
dc.identifier.wosid | 000345897600021 | - |
dc.identifier.bibliographicCitation | MOLECULAR BIOSYSTEMS, v.11, no.1, pp.218 - 222 | - |
dc.relation.isPartOf | MOLECULAR BIOSYSTEMS | - |
dc.citation.title | MOLECULAR BIOSYSTEMS | - |
dc.citation.volume | 11 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 218 | - |
dc.citation.endPage | 222 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | RESOLVED CIRCULAR-DICHROISM | - |
dc.subject.keywordPlus | ELECTRON-TRANSFER | - |
dc.subject.keywordPlus | PULSE-RADIOLYSIS | - |
dc.subject.keywordPlus | RESONANCE RAMAN | - |
dc.subject.keywordPlus | SPEED LIMIT | - |
dc.subject.keywordPlus | TRANSIENT ABSORPTION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | TIME | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.