Homogeneous generation of iDA neurons with high similarity to bona fide DA neurons using a drug inducible system
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, H. | - |
dc.contributor.author | Kim, H. | - |
dc.contributor.author | Yoo, J. | - |
dc.contributor.author | Lee, J. | - |
dc.contributor.author | Choi, H. | - |
dc.contributor.author | Baek, S. | - |
dc.contributor.author | Lee, C.J. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Lengner, C.J. | - |
dc.contributor.author | Sung, J.-S. | - |
dc.contributor.author | Kim, J. | - |
dc.date.accessioned | 2021-09-05T00:01:58Z | - |
dc.date.available | 2021-09-05T00:01:58Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/95961 | - |
dc.description.abstract | Recent work generating induced dopaminergic (iDA) neurons using direct lineage reprogramming potentially provides a novel platform for the study and treatment Parkinson's disease (PD). However, one of the most important issues for iDA-based applications is the degree to which iDA neurons resemble the molecular and functional properties of their endogenous DA neuron counterparts. Here we report that the homogeneity of the reprogramming gene expression system is critical for the generation of iDA neuron cultures that are highly similar to endogenous DA neurons. We employed an inducible system that carries iDA-inducing factors as defined transgenes for direct lineage reprogramming to iDA neurons. This system circumvents the need for viral transduction, enabling a more efficient and reproducible reprogramming process for the generation of genetically homogenous iDA neurons. We showed that this inducible system generates iDA neurons with high similarity to their bona fide in vivo counterparts in comparison to direct infection methods. Thus, our results suggest that homogenous expression of exogenous genes in direct lineage reprogramming is critical for the generation of high quality iDA neuron cultures, making such culture systems a valuable resource for iDA-based drug screening and, ultimately, potential therapeutic intervention in PD. © 2015 Elsevier Ltd. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Ltd | - |
dc.subject | Diagnosis | - |
dc.subject | Gene expression | - |
dc.subject | Culture systems | - |
dc.subject | Direct lineage reprogramming | - |
dc.subject | Expression system | - |
dc.subject | Functional properties | - |
dc.subject | Inducible systems | - |
dc.subject | Inducing factors | - |
dc.subject | Parkinson&apos | - |
dc.subject | s disease | - |
dc.subject | Therapeutic intervention | - |
dc.subject | Neurons | - |
dc.subject | animal cell | - |
dc.subject | animal experiment | - |
dc.subject | animal model | - |
dc.subject | Article | - |
dc.subject | cell lineage | - |
dc.subject | cell transplantation | - |
dc.subject | controlled study | - |
dc.subject | dopaminergic nerve cell | - |
dc.subject | embryo | - |
dc.subject | gene expression system | - |
dc.subject | male | - |
dc.subject | mouse | - |
dc.subject | nerve cell culture | - |
dc.subject | nonhuman | - |
dc.subject | Parkinson disease | - |
dc.subject | priority journal | - |
dc.subject | reproducibility | - |
dc.subject | transgene | - |
dc.subject | viral gene delivery system | - |
dc.subject | animal | - |
dc.subject | cell shape | - |
dc.subject | cytology | - |
dc.subject | dopaminergic nerve cell | - |
dc.subject | drug effects | - |
dc.subject | electrophysiology | - |
dc.subject | fibroblast | - |
dc.subject | hematopoietic stem cell | - |
dc.subject | metabolism | - |
dc.subject | nuclear reprogramming | - |
dc.subject | doxycycline | - |
dc.subject | enhanced green fluorescent protein | - |
dc.subject | green fluorescent protein | - |
dc.subject | homeodomain protein | - |
dc.subject | transcription factor | - |
dc.subject | transcription factor Pitx3 | - |
dc.subject | Animals | - |
dc.subject | Cell Lineage | - |
dc.subject | Cell Shape | - |
dc.subject | Cellular Reprogramming | - |
dc.subject | Dopaminergic Neurons | - |
dc.subject | Doxycycline | - |
dc.subject | Electrophysiological Phenomena | - |
dc.subject | Fibroblasts | - |
dc.subject | Green Fluorescent Proteins | - |
dc.subject | Hematopoietic Stem Cells | - |
dc.subject | Homeodomain Proteins | - |
dc.subject | Mice | - |
dc.subject | Transcription Factors | - |
dc.title | Homogeneous generation of iDA neurons with high similarity to bona fide DA neurons using a drug inducible system | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, C.J. | - |
dc.identifier.doi | 10.1016/j.biomaterials.2015.09.002 | - |
dc.identifier.scopusid | 2-s2.0-84943245228 | - |
dc.identifier.bibliographicCitation | Biomaterials, v.72, pp.152 - 162 | - |
dc.relation.isPartOf | Biomaterials | - |
dc.citation.title | Biomaterials | - |
dc.citation.volume | 72 | - |
dc.citation.startPage | 152 | - |
dc.citation.endPage | 162 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | Diagnosis | - |
dc.subject.keywordPlus | Gene expression | - |
dc.subject.keywordPlus | Culture systems | - |
dc.subject.keywordPlus | Direct lineage reprogramming | - |
dc.subject.keywordPlus | Expression system | - |
dc.subject.keywordPlus | Functional properties | - |
dc.subject.keywordPlus | Inducible systems | - |
dc.subject.keywordPlus | Inducing factors | - |
dc.subject.keywordPlus | Parkinson&apos | - |
dc.subject.keywordPlus | s disease | - |
dc.subject.keywordPlus | Therapeutic intervention | - |
dc.subject.keywordPlus | Neurons | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | animal experiment | - |
dc.subject.keywordPlus | animal model | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | cell lineage | - |
dc.subject.keywordPlus | cell transplantation | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | dopaminergic nerve cell | - |
dc.subject.keywordPlus | embryo | - |
dc.subject.keywordPlus | gene expression system | - |
dc.subject.keywordPlus | male | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nerve cell culture | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | Parkinson disease | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | reproducibility | - |
dc.subject.keywordPlus | transgene | - |
dc.subject.keywordPlus | viral gene delivery system | - |
dc.subject.keywordPlus | animal | - |
dc.subject.keywordPlus | cell shape | - |
dc.subject.keywordPlus | cytology | - |
dc.subject.keywordPlus | dopaminergic nerve cell | - |
dc.subject.keywordPlus | drug effects | - |
dc.subject.keywordPlus | electrophysiology | - |
dc.subject.keywordPlus | fibroblast | - |
dc.subject.keywordPlus | hematopoietic stem cell | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | nuclear reprogramming | - |
dc.subject.keywordPlus | doxycycline | - |
dc.subject.keywordPlus | enhanced green fluorescent protein | - |
dc.subject.keywordPlus | green fluorescent protein | - |
dc.subject.keywordPlus | homeodomain protein | - |
dc.subject.keywordPlus | transcription factor | - |
dc.subject.keywordPlus | transcription factor Pitx3 | - |
dc.subject.keywordPlus | Animals | - |
dc.subject.keywordPlus | Cell Lineage | - |
dc.subject.keywordPlus | Cell Shape | - |
dc.subject.keywordPlus | Cellular Reprogramming | - |
dc.subject.keywordPlus | Dopaminergic Neurons | - |
dc.subject.keywordPlus | Doxycycline | - |
dc.subject.keywordPlus | Electrophysiological Phenomena | - |
dc.subject.keywordPlus | Fibroblasts | - |
dc.subject.keywordPlus | Green Fluorescent Proteins | - |
dc.subject.keywordPlus | Hematopoietic Stem Cells | - |
dc.subject.keywordPlus | Homeodomain Proteins | - |
dc.subject.keywordPlus | Mice | - |
dc.subject.keywordPlus | Transcription Factors | - |
dc.subject.keywordAuthor | Direct lineage reprogramming | - |
dc.subject.keywordAuthor | Gene expression system | - |
dc.subject.keywordAuthor | Induced neurons | - |
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