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A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome analysis

Authors
Lee, HangyeoreMun, Dong-GiBae, JingiKim, HokeunOh, Se YeonPark, Young SooLee, Jae-HyukLee, Sang-Won
Issue Date
2015
Publisher
ROYAL SOC CHEMISTRY
Citation
ANALYST, v.140, no.16, pp.5700 - 5706
Indexed
SCIE
SCOPUS
Journal Title
ANALYST
Volume
140
Number
16
Start Page
5700
End Page
5706
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/96208
DOI
10.1039/c5an00639b
ISSN
0003-2654
Abstract
We report a new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system. The system employs only two nano-volume switching valves (a two-position four port valve and a two-position ten port valve) that direct solvent flows from two binary nano-pumps for parallel operation of two analytical columns and two solid phase extraction (SPE) columns. Despite the simple design, the sDO-UHPLC offers many advantageous features that include high duty cycle, back flushing sample injection for fast and narrow zone sample injection, online desalting, high separation resolution and high intra/inter-column reproducibility. This system was applied to analyze proteome samples not only in high throughput deep proteome profiling experiments but also in high throughput MRM experiments.
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