A quantitative microfluidic angiogenesis screen for studying anti-angiogenic therapeutic drugs
DC Field | Value | Language |
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dc.contributor.author | Kim, Choong | - |
dc.contributor.author | Kasuya, Junichi | - |
dc.contributor.author | Jeon, Jessie | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Kamm, Roger D. | - |
dc.date.accessioned | 2021-09-05T01:08:27Z | - |
dc.date.available | 2021-09-05T01:08:27Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96295 | - |
dc.description.abstract | Anti-angiogenic therapy, which suppresses tumor growth by disrupting oxygen and nutrient supply from blood to the tumor, is now widely accepted as a treatment for cancer. To investigate the mechanisms of action of these anti-angiogenesis drugs, new three dimensional (3D) cell culture-based drug screening models are increasingly employed. However, there is no in vitro high-throughput screening (HTS) angiogenesis assay that can provide uniform culture conditions for the quantitative assessment of physiological responses to chemoattractant reagents under various concentrations of anti-angiogenesis drugs. Here we describe a method for screening and quantifying the vascular endothelial growth factor (VEGF)-induced chemotactic response on human umbilical vein endothelial cells (HUVECs) cultured with different concentrations of bortezomib, a selective 26S proteasome inhibitor. With this quantitative microfluidic angiogenesis screen (QMAS), we demonstrate that bortezomib-induced endothelial cell death is preceded by a series of morphological changes that develop over several days. We also explore the mechanisms by which bortezomib can inhibit angiogenesis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | IN-VITRO | - |
dc.subject | CELL-MIGRATION | - |
dc.subject | TUMOR SPHEROIDS | - |
dc.subject | BORTEZOMIB | - |
dc.subject | CULTURE | - |
dc.subject | MORPHOGENESIS | - |
dc.subject | THERAPIES | - |
dc.subject | SCAFFOLDS | - |
dc.subject | APOPTOSIS | - |
dc.subject | PLATFORM | - |
dc.title | A quantitative microfluidic angiogenesis screen for studying anti-angiogenic therapeutic drugs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Seok | - |
dc.identifier.doi | 10.1039/c4lc00866a | - |
dc.identifier.scopusid | 2-s2.0-84915793469 | - |
dc.identifier.wosid | 000346478100035 | - |
dc.identifier.bibliographicCitation | LAB ON A CHIP, v.15, no.1, pp.301 - 310 | - |
dc.relation.isPartOf | LAB ON A CHIP | - |
dc.citation.title | LAB ON A CHIP | - |
dc.citation.volume | 15 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 301 | - |
dc.citation.endPage | 310 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CELL-MIGRATION | - |
dc.subject.keywordPlus | TUMOR SPHEROIDS | - |
dc.subject.keywordPlus | BORTEZOMIB | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | MORPHOGENESIS | - |
dc.subject.keywordPlus | THERAPIES | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | PLATFORM | - |
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