Seed-specific expression of seven Arabidopsis promoters
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Hee-Jeong | - |
dc.contributor.author | Choi, Jun Young | - |
dc.contributor.author | Shin, Hyun Young | - |
dc.contributor.author | Bae, Jung-Myung | - |
dc.contributor.author | Shin, Jeong Sheop | - |
dc.date.accessioned | 2021-09-05T02:08:48Z | - |
dc.date.available | 2021-09-05T02:08:48Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-12-10 | - |
dc.identifier.issn | 0378-1119 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96511 | - |
dc.description.abstract | Seeds contain storage compounds, from various carbohydrates to proteins and lipids, which are synthesized during seed development. For the purposes of many plant researches or commercial applications, developing promoter systems expressing specifically in seeds or in particular constituents or tissues/compartments of seeds are indispensable. To screen genes dominantly or specifically expressed in seed tissues, we analyzed Arabidopsis ATH1 microarray data open to the public. Thirty-two candidate genes were selected and their expressions in seed tissues were confirmed by RT-PCR. Finally, seven genes were selected for promoter analysis. The promoters of seven genes were cloned into pBI101 vector and transformed into Arabidopsis to assay histochemical beta-glucuronidase (GUS) activity. We found that Pro-at3g03230 promoter drove GUS expression in a chalazal endosperm, Pro-at4g27530:GUS expressed in both chalazal endosperm and embryo, Pro-at4g31830 accelerated GUS expression both in radicle and procambium, Pro-at5g10120 and Pro-at5g16460 drove GUS expression uniquely in embryo, Pro-at5g53100:GUS expressed only in endosperm, and Pro-at5g54000 promoted GUS expression in both embryo and inner integument. These promoters can be used for expressing any genes in specific seed tissues for practical application. (C) 2014 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | THALIANA | - |
dc.subject | GENE | - |
dc.title | Seed-specific expression of seven Arabidopsis promoters | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Jeong Sheop | - |
dc.identifier.doi | 10.1016/j.gene.2014.09.051 | - |
dc.identifier.scopusid | 2-s2.0-84925224582 | - |
dc.identifier.wosid | 000344426300003 | - |
dc.identifier.bibliographicCitation | GENE, v.553, no.1, pp.17 - 23 | - |
dc.relation.isPartOf | GENE | - |
dc.citation.title | GENE | - |
dc.citation.volume | 553 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 17 | - |
dc.citation.endPage | 23 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | THALIANA | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | ATH1 microarray | - |
dc.subject.keywordAuthor | Seed tissue-specific | - |
dc.subject.keywordAuthor | Promoters | - |
dc.subject.keywordAuthor | Arabidopsis thaliana | - |
dc.subject.keywordAuthor | Gene expression | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.