Protective Effect of Metformin on Gentamicin-Induced Vestibulotoxicity in Rat Primary Cell Culture
DC Field | Value | Language |
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dc.contributor.author | Lee, Ji Young | - |
dc.contributor.author | Lee, Se Hee | - |
dc.contributor.author | Chang, Ji Won | - |
dc.contributor.author | Song, Jae-June | - |
dc.contributor.author | Jung, Hak Hyun | - |
dc.contributor.author | Im, Gi Jung | - |
dc.date.accessioned | 2021-09-05T02:24:46Z | - |
dc.date.available | 2021-09-05T02:24:46Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.issn | 1976-8710 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96593 | - |
dc.description.abstract | Objectives. One of the antidiabetic drugs, metformin, have shown that it prevented oxidative stress-induced death in several cell types through a mechanism involving the opening of the permeability transition pore and cytochrome c release. Thus, it is possible that the antioxidative effect of metformin can also serve as protection against gentamicin-induced cytotoxicity related to reactive oxygen species (ROS). The aim of this study was to examine the protective effect of metformin on gentamicin-induced vestibulotoxicity in primary cell culture derived from rat utricle. Methods. For vestibular primary cell culture, rat utricles were dissected and incubated. Gentamicin-induced cytotoxicity was measured in both the auditory and vestibular cells. To examine the effects of metformin on gentamicin-induced cytotoxicity in the primary cell culture, the cells were pretreated with metformin at a concentration of 1 mM for 24 hours, and then exposed to 2.5 mM gentamicin for 48 hours. The intracellular ROS level was measured using a fluorescent dye, and also measured using a FACScan flow cytometer. Intracellular calcium levels in the vestibular cells were measured with calcium imaging using Fura-2 AM. Results. Vestibular cells were more sensitive to gentamicin-induced cytotoxicity than auditory hair cells. Metformin protects against gentamicin-induced cytotoxicity in vestibular cells. Metformin significantly reduced a gentamicin-induced increase in ROS. and also reduced an increase in intracellular calcium concentrations in gentamicin-induced cytotoxicity. Conclusion. Metformin significantly reduced a gentamicin-induced increase in ROS, stabilized the intracellular calcium concentration, and inhibited gentamicin-induced apoptosis. Thus, Metformin showed protective effect on gentamicin-induced cytotoxicity in vestibular primary cell culture. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC OTORHINOLARYNGOL | - |
dc.subject | MITOCHONDRIAL PERMEABILITY TRANSITION | - |
dc.subject | ANTIDIABETIC DRUG METFORMIN | - |
dc.subject | MENIERES-DISEASE | - |
dc.subject | INTRATYMPANIC GENTAMICIN | - |
dc.subject | ENDOTHELIAL-CELLS | - |
dc.subject | IN-VITRO | - |
dc.subject | OTOTOXICITY | - |
dc.subject | DEATH | - |
dc.subject | HYPERGLYCEMIA | - |
dc.subject | APOPTOSIS | - |
dc.title | Protective Effect of Metformin on Gentamicin-Induced Vestibulotoxicity in Rat Primary Cell Culture | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Jae-June | - |
dc.contributor.affiliatedAuthor | Jung, Hak Hyun | - |
dc.contributor.affiliatedAuthor | Im, Gi Jung | - |
dc.identifier.doi | 10.3342/ceo.2014.7.4.286 | - |
dc.identifier.scopusid | 2-s2.0-84911392333 | - |
dc.identifier.wosid | 000346221200009 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, v.7, no.4, pp.286 - 294 | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY | - |
dc.citation.title | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY | - |
dc.citation.volume | 7 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 286 | - |
dc.citation.endPage | 294 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001936554 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.subject.keywordPlus | MITOCHONDRIAL PERMEABILITY TRANSITION | - |
dc.subject.keywordPlus | ANTIDIABETIC DRUG METFORMIN | - |
dc.subject.keywordPlus | MENIERES-DISEASE | - |
dc.subject.keywordPlus | INTRATYMPANIC GENTAMICIN | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | OTOTOXICITY | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | HYPERGLYCEMIA | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordAuthor | Labyrinth vestibule | - |
dc.subject.keywordAuthor | Gentamicins | - |
dc.subject.keywordAuthor | Metformin | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
dc.subject.keywordAuthor | Calcium | - |
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