Evaluation of penetration of nanocarriers into red pepper leaf using confocal laser scanning microscopy
- Authors
- Minh-Hiep Nguyen; Lee, Ji-Sun; Hwang, In-Cheon; Park, Huyn-Jin
- Issue Date
- 12월-2014
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Chitosan; Deltamethrin; Nanocarriers; Penetration; Zeta-potential; Leaf
- Citation
- CROP PROTECTION, v.66, pp.61 - 66
- Indexed
- SCIE
SCOPUS
- Journal Title
- CROP PROTECTION
- Volume
- 66
- Start Page
- 61
- End Page
- 66
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/96706
- DOI
- 10.1016/j.cropro.2014.08.016
- ISSN
- 0261-2194
- Abstract
- Penetration of nanocarriers into red pepper leaf was evaluated with the aim of identifying a novel way to enhance the efficacy of applied pesticides on the field. In addition, the effect of surface charge of the nanocarriers on penetration was also studied. Particularly, corn oil-nanoemulsions (NE) and chitosan coated NE (CH-NE) were successfully prepared with a high encapsulation efficiency, a high payload, a very small size, a small polydispersity index, and a high zeta potential. However, after being coated with chitosan, the zeta potential of NE changed from a negative charge to a positive charge. Penetration experiments were also carried out using a Franz diffusion cell followed by visualization using confocal laser scanning microscopy. The images of the vertical sections illustrated that penetration of nanocarriers (NE and CH-NE) into the red pepper leaf occurred very quickly. Nanocarriers fully penetrated the whole leaf after 60 min. Moreover, multi-depth images that paralleled the leaf surface (horizontal visualization) together with removal of plant-autofluorescent emissions and three-dimensional graphs describing the penetration of NE and CH-NE demonstrated that the negatively charged nanocarriers (NE) had a higher penetration rate compared to that of the positively charged nanocarriers (CH-NE). (C) 2014 Elsevier Ltd. All rights reserved.
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