Global identification of target recognition and cleavage by the Microprocessor in human ES cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seong, Youngmo | - |
dc.contributor.author | Lim, Do-Hwan | - |
dc.contributor.author | Kim, Augustine | - |
dc.contributor.author | Seo, Jae Hong | - |
dc.contributor.author | Lee, Young Sik | - |
dc.contributor.author | Song, Hoseok | - |
dc.contributor.author | Kwon, Young-Soo | - |
dc.date.accessioned | 2021-09-05T02:58:30Z | - |
dc.date.available | 2021-09-05T02:58:30Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-11-10 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96782 | - |
dc.description.abstract | The Microprocessor plays an essential role in canonical miRNA biogenesis by facilitating cleavage of stem-loop structures in primary transcripts to yield pre-miRNAs. Although miRNA biogenesis has been extensively studied through biochemical and molecular genetic approaches, it has yet to be addressed to what extent the current miRNA biogenesis models hold true in intact cells. To address the issues of in vivo recognition and cleavage by the Microprocessor, we investigate RNAs that are associated with DGCR8 and Drosha by using immunoprecipitation coupled with next-generation sequencing. Here, we present global protein-RNA interactions with unprecedented sensitivity and specificity. Our data indicate that precursors of canonical miRNAs and miRNA-like hairpins are the major substrates of the Microprocessor. As a result of specific enrichment of nascent cleavage products, we are able to pinpoint the Microprocessor-mediated cleavage sites per se at single-nucleotide resolution. Unexpectedly, a 2-nt 3' overhang invariably exists at the ends of cleaved bases instead of nascent pre-miRNAs. Besides canonical miRNA precursors, we find that two novel miRNA-like structures embedded in mRNAs are cleaved to yield pre-miRNA-like hairpins, uncoupled from miRNA maturation. Our data provide a framework for in vivo Microprocessor-mediated cleavage and a foundation for experimental and computational studies on miRNA biogenesis in living cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | PRIMARY MICRORNA | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | 5&apos | - |
dc.subject | END | - |
dc.subject | EXPRESSION | - |
dc.subject | DROSHA | - |
dc.subject | EFFICIENT | - |
dc.subject | DICER | - |
dc.subject | MIRNA | - |
dc.subject | BIOGENESIS | - |
dc.subject | HUNDREDS | - |
dc.title | Global identification of target recognition and cleavage by the Microprocessor in human ES cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seo, Jae Hong | - |
dc.contributor.affiliatedAuthor | Lee, Young Sik | - |
dc.contributor.affiliatedAuthor | Song, Hoseok | - |
dc.identifier.doi | 10.1093/nar/gku957 | - |
dc.identifier.wosid | 000347693200043 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.42, no.20, pp.12806 - 12821 | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 42 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 12806 | - |
dc.citation.endPage | 12821 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | PRIMARY MICRORNA | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | 5&apos | - |
dc.subject.keywordPlus | END | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DROSHA | - |
dc.subject.keywordPlus | EFFICIENT | - |
dc.subject.keywordPlus | DICER | - |
dc.subject.keywordPlus | MIRNA | - |
dc.subject.keywordPlus | BIOGENESIS | - |
dc.subject.keywordPlus | HUNDREDS | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.