Gln-362 of Angiopoietin-2 Mediates Migration of Tumor and Endothelial Cells through Association with alpha 5 beta 1 Integrin
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyo Seon | - |
dc.contributor.author | Oh, Seung Ja | - |
dc.contributor.author | Lee, Kwang-Hoon | - |
dc.contributor.author | Lee, Yoon-Sook | - |
dc.contributor.author | Ko, Eun | - |
dc.contributor.author | Kim, Kyung Eun | - |
dc.contributor.author | Kim, Hyung-chan | - |
dc.contributor.author | Kim, Seokkyun | - |
dc.contributor.author | Song, Paul H. | - |
dc.contributor.author | Kim, Yong-In | - |
dc.contributor.author | Kim, Chungho | - |
dc.contributor.author | Han, Sangyeul | - |
dc.date.accessioned | 2021-09-05T02:59:10Z | - |
dc.date.available | 2021-09-05T02:59:10Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-11-07 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96786 | - |
dc.description.abstract | Angiopoietin-2 (Ang-2) not only regulates angiogenesis by binding to its well known receptor Tie2 on endothelial cells but also controls sprouting of Tie2-negative angiogenic endothelial cells and invasion of Tie2-negative non-endothelial cells by binding to integrins. However, the molecular mechanism of the Ang-2/ integrin association has been unclear. In this study, we found that the Gln-362 residue of Ang-2 was essential for binding to alpha 5 beta 1 integrin. A Q362E Ang-2 mutant, which still bound to Tie2, failed to associate with alpha 5 beta 1 integrin and was unable to activate the integrin downstream signaling of focal adhesion kinase. In addition, unlike wild-type Ang-2, the Q362E Ang-2 mutant was defective in mediating invasion of Tie2-negative glioma or Tie2-positive endothelial cells. Furthermore, the tailpiece domain of the alpha 5 subunit in alpha 5 beta 1 integrin was critical for binding to Ang-2. Taken together, these results provide a novel insight into the mechanism of integrin regulation by Ang-2, which contributes to tumor invasion and endothelial cell migration in a Tie2-independent manner. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | MATRIX METALLOPROTEASE-2 | - |
dc.subject | TIE2 RECEPTOR | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | CANCER | - |
dc.subject | PATHWAY | - |
dc.subject | INVASION | - |
dc.subject | ALPHA-V-BETA-3 | - |
dc.subject | VASCULATURE | - |
dc.subject | METASTASIS | - |
dc.subject | EXPRESSION | - |
dc.title | Gln-362 of Angiopoietin-2 Mediates Migration of Tumor and Endothelial Cells through Association with alpha 5 beta 1 Integrin | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chungho | - |
dc.identifier.doi | 10.1074/jbc.M114.572594 | - |
dc.identifier.scopusid | 2-s2.0-84909619196 | - |
dc.identifier.wosid | 000344549900037 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.289, no.45, pp.31330 - 31340 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 289 | - |
dc.citation.number | 45 | - |
dc.citation.startPage | 31330 | - |
dc.citation.endPage | 31340 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEASE-2 | - |
dc.subject.keywordPlus | TIE2 RECEPTOR | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordPlus | ALPHA-V-BETA-3 | - |
dc.subject.keywordPlus | VASCULATURE | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
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