Immobilization of acetyl xylan esterase on modified graphite oxide and utilization to peracetic acid production
- Authors
- Yoo, Hah Young; Lee, Ja Hyun; Suh, Young Joon; Kim, Sung Bong; Park, Seung-Moon; Kim, Seung Wook
- Issue Date
- 11월-2014
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- acetyl xylan esterase; peracetic acid; enzyme immobilization; graphite oxide
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.19, no.6, pp.1042 - 1047
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 19
- Number
- 6
- Start Page
- 1042
- End Page
- 1047
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/96897
- DOI
- 10.1007/s12257-014-0298-8
- ISSN
- 1226-8372
- Abstract
- In the previous work, acetyl xylan esterase (AXE) of Aspergillus ficcum was successfully produced in Pichia pastoris as host. In this study, the recombinant AXE was immobilized on graphite oxide and used for the production of peracetic acid. Immobilization efficiency was enhanced by modifying graphite oxide via surface functionalization. The conditions for enzyme immobilization were also investigated and the optimal conditions were determined as 4a"integral of temperature, 24 h of reaction time and pH 7. The activity of immobilized AXE was found to be 62.53 U/g-support. With the immobilized AXE, about 134 mM of peracetic acid was produced under 37a"integral of temperature and 30 min of reaction time. Enzyme activity remained at > 50% of the original after 10 production cycles.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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