Histamine Promotes the Release of Interleukin-6 via the H1R/p38 and NF-kappa B Pathways in Nasal Fibroblasts
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Ii-Ho | - |
dc.contributor.author | Um, Ji-Young | - |
dc.contributor.author | Cho, Jung-Sun | - |
dc.contributor.author | Lee, Seung Hoon | - |
dc.contributor.author | Lee, Sang Hag | - |
dc.contributor.author | Lee, Heung-Man | - |
dc.date.accessioned | 2021-09-05T03:31:01Z | - |
dc.date.available | 2021-09-05T03:31:01Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-11 | - |
dc.identifier.issn | 2092-7355 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96942 | - |
dc.description.abstract | Purpose: Based on the close relationship between histamine and interleukin 6 (IL-6), we hypothesized that histamine may regulate the production of cytokines, such as IL-6, during allergic inflammation. Here, we examined the role of histamine in IL-6 production and histamine receptor activity in nasal fibroblasts, along with the mechanisms underlying these effects. Methods: Experiments were performed using nasal fibroblasts from 8 normal patients. RT-PCR was used to identify the major histamine receptors expressed in nasal fibroblasts. Fibroblasts were then treated with histamine with or without histamine-receptor antagonists, and monitored for IL-6 production using an ELISA. Four potential downstream signaling molecules, p38, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and NF-kappa B, were evaluated by Western blot, and a luciferase reporter assay. Results: Elevated expression was seen for all histamine receptors, with IL-6 protein levels increasing significantly following histamine stimulation. Among the histamine-receptor specific antagonists, only the H1R antagonist significantly decreased IL-6 production in histaminestimulated nasal fibroblasts. Histamine increased the expression level of phosphorylated p38 (pp38), pERK, and pJNK, as well as NF-K kappa B induction. The H1R antagonist actively suppressed pp38 and NF-kappa B expression in histamine-induced nasal fibroblasts, but not pERK and pJNK. The p38 inhibitor strongly attenuated IL-6 production in histamine-stimulated nasal fibroblasts. Conclusions: The data presented here suggest that antihistamines may be involved in the regulation of cytokines, such as IL-6, due to the role of histamine as an inflammatory mediator in nasal fibroblasts. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN ACAD ASTHMA ALLERGY & CLINICAL IMMUNOLOGY | - |
dc.subject | ALLERGIC RHINITIS | - |
dc.subject | PROINFLAMMATORY CYTOKINES | - |
dc.subject | IL-6 PRODUCTION | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | RECEPTORS | - |
dc.subject | MEDICINE | - |
dc.subject | DISEASE | - |
dc.subject | ASTHMA | - |
dc.subject | CELLS | - |
dc.title | Histamine Promotes the Release of Interleukin-6 via the H1R/p38 and NF-kappa B Pathways in Nasal Fibroblasts | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Ii-Ho | - |
dc.contributor.affiliatedAuthor | Lee, Seung Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Sang Hag | - |
dc.contributor.affiliatedAuthor | Lee, Heung-Man | - |
dc.identifier.doi | 10.4168/aair.2014.6.6.567 | - |
dc.identifier.scopusid | 2-s2.0-84909593222 | - |
dc.identifier.wosid | 000350832900013 | - |
dc.identifier.bibliographicCitation | ALLERGY ASTHMA & IMMUNOLOGY RESEARCH, v.6, no.6, pp.567 - 572 | - |
dc.relation.isPartOf | ALLERGY ASTHMA & IMMUNOLOGY RESEARCH | - |
dc.citation.title | ALLERGY ASTHMA & IMMUNOLOGY RESEARCH | - |
dc.citation.volume | 6 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 567 | - |
dc.citation.endPage | 572 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001926920 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Allergy | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Allergy | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | ALLERGIC RHINITIS | - |
dc.subject.keywordPlus | PROINFLAMMATORY CYTOKINES | - |
dc.subject.keywordPlus | IL-6 PRODUCTION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | MEDICINE | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Nose | - |
dc.subject.keywordAuthor | fibroblast | - |
dc.subject.keywordAuthor | histamine | - |
dc.subject.keywordAuthor | IL-6 | - |
dc.subject.keywordAuthor | allergic rhinitis | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.