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Highly Sensitive Immunoassay for the Diagnosis of Acute Myocardial Infarction Using Silica Spheres Encapsulating a Quantum Dot Layer

Authors
Han, HyojeongPyun, Jae-ChulYoo, HyeinSeo, Hong SeogJung, Byung HwaYoo, Young SookWoo, KyoungjaKang, Min-Jung
Issue Date
21-10월-2014
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.86, no.20, pp.10157 - 10163
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL CHEMISTRY
Volume
86
Number
20
Start Page
10157
End Page
10163
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/97064
DOI
10.1021/ac502412x
ISSN
0003-2700
Abstract
Commercial ELISA kits for substance P (SubP), which are helpful for the clinical diagnosis of acute myocardial infarction, are limited in efficacy because of low sensitivity. A highly sensitive immunoassay was developed using silica spheres encapsulating a quantum dot-layer (SQS) and labeling antibodies, on a Parylene A-modified plate. The high sensitivity was possible by taking advantage of the enhanced photoluminescence of the SQS and dense immobilization of SubP on a Parylene A-modified plate. Glutaraldehyde was used for cross-linking of SQS to the anti-SubP antibody and SubP to the Parylene A coating. The SQS-linked immunosorbent assay (SQSLISA) was optimized and validated. The dynamic range for the assay was 1-10000 pg/mL with a linear correlation factor of 0.9992 when the competitive SQSLISA was employed. The intra- and interday accuracies were 93-100% and 87-122%, respectively. The reproducibility was lower than 11%. The developed method was applied to clinical samples collected from healthy controls (n = 30) and acute myocardial infarction (n = 16) and it displayed a high correlation with the commercial ELISA kit, with a limit of detection that was 30-fold lower. Clinical sample analysis confirmed that SubP is a promising diagnostic marker for acute myocardial infarction. The SQSLISA is expected to be a practical and useful assay tool.
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